The genomes of attenuated and virulent poliovirus strains differ in their in vitro translation efficiencies.

@article{Svitkin1985TheGO,
  title={The genomes of attenuated and virulent poliovirus strains differ in their in vitro translation efficiencies.},
  author={Yuri V. Svitkin and Svetlana V. Maslova and Vadim I Agol},
  journal={Virology},
  year={1985},
  volume={147 2},
  pages={243-52}
}
In mRNA-dependent extracts of Krebs-2 cells, RNAs from attenuated strains of poliovirus type 1 and type 3 exhibited diminished template activity as compared to RNAs from the respective virulent counterparts. This defect appeared to be due to the impaired initiation of viral polyprotein synthesis as evidenced by a relatively low level of accumulation of polypeptide 1a (which corresponds to an NH2-terminal region of the polyprotein) in samples programmed with RNAs from attenuated strains. In… CONTINUE READING