The genome structure of Pseudomonas putida: high-resolution mapping and microarray analysis.

Abstract

As part of a collaborative project aimed at sequencing and functionally analysing the entire genome of Pseudomonas putida strain KT2440, a physical clone map was produced as an initial resource. To this end, a high-coverage cosmid library was arrayed and ordered by clone hybridizations. Restriction fragments generated by rare-cutting enzymes and plasmids containing the rrn operon and 23S rDNA of Pseudomonas aeruginosa were used as probes and, parts of the cosmids were end-sequenced. This provided the information necessary for merging and comparing the macro-restriction map, cosmid clone order and sequence information, thereby assuring co-linearity of the eventual sequence assembly with the actual genome. A tiling path of clones was selected, from the shotgun clones used for sequencing, for the production of DNA microarrays that represent the entire genome including its non-coding portions.

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Cite this paper

@article{Stjepandi2002TheGS, title={The genome structure of Pseudomonas putida: high-resolution mapping and microarray analysis.}, author={Diana Stjepandi{\'c} and Christian Weinel and Helmut Hilbert and Hean L Koo and Frank Diehl and Karen E. Nelson and Burkhard T{\"{u}mmler and J{\"{o}rg D. Hoheisel}, journal={Environmental microbiology}, year={2002}, volume={4 12}, pages={819-23} }