The genome sequence of the plant pathogen Xylella fastidiosa. The Xylella fastidiosa Consortium of the Organization for Nucleotide Sequencing and Analysis.

Abstract

Xylella fastidiosa is a fastidious, xylem-limited bacterium that causes a range of economically important plant diseases. Here we report the complete genome sequence of X. fastidiosa clone 9a5c, which causes citrus variegated chlorosis--a serious disease of orange trees. The genome comprises a 52.7% GC-rich 2,679,305-base-pair (bp) circular chromosome and two plasmids of 51,158 bp and 1,285 bp. We can assign putative functions to 47% of the 2,904 predicted coding regions. Efficient metabolic functions are predicted, with sugars as the principal energy and carbon source, supporting existence in the nutrient-poor xylem sap. The mechanisms associated with pathogenicity and virulence involve toxins, antibiotics and ion sequestration systems, as well as bacterium-bacterium and bacterium-host interactions mediated by a range of proteins. Orthologues of some of these proteins have only been identified in animal and human pathogens; their presence in X. fastidiosa indicates that the molecular basis for bacterial pathogenicity is both conserved and independent of host. At least 83 genes are bacteriophage-derived and include virulence-associated genes from other bacteria, providing direct evidence of phage-mediated horizontal gene transfer.

0200400'01'03'05'07'09'11'13'15'17
Citations per Year

2,882 Citations

Semantic Scholar estimates that this publication has 2,882 citations based on the available data.

See our FAQ for additional information.

Cite this paper

@article{Simpson2000TheGS, title={The genome sequence of the plant pathogen Xylella fastidiosa. The Xylella fastidiosa Consortium of the Organization for Nucleotide Sequencing and Analysis.}, author={A J Simpson and F C Reinach and P Arruda and F A Abreu and M Acencio and R Alvarenga and L M Alves and J E Araya and G S Baia and C S Baptista and M H Barros and E D Bonaccorsi and S Bordin and J M Bov{\'e} and M R Briones and M R Bueno and A A Camargo and L E Camargo and D M Carraro and H Carrer and N B Colauto and C Colombo and F F Costa and M C Costa and C M Costa-Neto and L L Coutinho and M Cristofani and E Dias-Neto and C Docena and H El-Dorry and A P Facincani and A J Ferreira and V C Ferreira and J A Ferro and J S Fraga and S C França and M C Franco and M Frohme and L R Furlan and M Garnier and G H Goldman and M H Goldman and S L Gomes and A Gruber and P L Ho and J D Hoheisel and M L Junqueira and E L Kemper and J P Kitajima and J E Krieger and E E Kuramae and F Laigret and M R Lambais and L C Leite and E G Lemos and M V Lemos and S A Lopes and C R Lopes and J A Machado and M A Machado and A M Madeira and H M Madeira and C L Marino and M V Marques and E A Martins and E M Martins and A Y Matsukuma and C F Menck and E C Miracca and C Y Miyaki and C B Monteriro-Vitorello and D H Moon and M A Nagai and A L Nascimento and L E Netto and A Nhani and F G Nobrega and L R Nunes and M A Oliveira and M C de Oliveira and R C de Oliveira and D A Palmieri and A Paris and B R Peixoto and G A Pereira and H A Pereira and J B Pesquero and R B Quaggio and P G Roberto and V Rodrigues and A J de M Rosa and V E de Rosa and R G de S{\'a} and R V Santelli and H E Sawasaki and A C da Silva and A M da Silva and F R da Silva and W A da Silva and J F da Silveira and M L Silvestri and W J Siqueira and A A de Souza and A P de Souza and M F Terenzi and D Truffi and S M Tsai and M H Tsuhako and H Vallada and M A Van Sluys and S Verjovski-Almeida and A L Vettore and M A Zago and M Zatz and J Meidanis and J C Setubal}, journal={Nature}, year={2000}, volume={406 6792}, pages={151-9} }