The genome sequence of the filamentous fungus Neurospora crassa

  title={The genome sequence of the filamentous fungus Neurospora crassa},
  author={James E. Galagan and Sarah E. Calvo and Katherine A. Borkovich and Eric U. Selker and Nick D Read and David B. Jaffe and William W. Fitzhugh and Li‐Jun Ma and Sergei Smirnov and Seth Purcell and Bushra Rehman and Timothy Elkins and Reinhard Engels and Shunguang Wang and Cydney B. Nielsen and Jonathan Butler and Matthew G. Endrizzi and D. W. Qui and Peter Ianakiev and Deborah Bell-Pedersen and Mary Anne Nelson and Margaret Werner-Washburne and Claude P. Selitrennikoff and John A. Kinsey and Edward L. Braun and Alex Zelter and Ulrich Schulte and Gregory O. Kothe and Gregory Jedd and Werner Mewes and Chuck Staben and Edward M. Marcotte and David Greenberg and Alice Roy and Karen Foley and Jerome W. Naylor and Nicole Stange-thomann and Robert Barrett and Sante Gnerre and Michael Kamal and Manolis Kamvysselis and Evan Mauceli and C. Bielke and Stephen Rudd and Dmitrij Frishman and Svetlana Kry{\vs}tofov{\'a} and Carolyn G. Rasmussen and Robert L. Metzenberg and David D. Perkins and Scott Kroken and Carlo Cogoni and Giuseppe Macino and David E. A. Catcheside and Weixi Li and Robert J. Pratt and Stephen A Osmani and Colin P. C. DeSouza and Louis Glass and Marc J. Orbach and J. Andrew Berglund and Rodger B. Voelker and Oded Yarden and Michael D. Plamann and Stephan Seiler and Jay C. Dunlap and Alan Radford and Rodolfo Aramayo and Donald O. Natvig and Lisa A. Alex and Gertrud Mannhaupt and Daniel J. Ebbole and Michael Freitag and Ian T. Paulsen and Matthew S. Sachs and Eric S. Lander and Chad Nusbaum and Bruce W. Birren},
Neurospora crassa is a central organism in the history of twentieth-century genetics, biochemistry and molecular biology. [] Key Result The approximately 40-megabase genome encodes about 10,000 protein-coding genes—more than twice as many as in the fission yeast Schizosaccharomyces pombe and only about 25% fewer than in the fruitfly Drosophila melanogaster.

Lessons from the Genome Sequence of Neurospora crassa: Tracing the Path from Genomic Blueprint to Multicellular Organism

An analysis of over 1,100 of the ∼10,000 predicted proteins encoded by the genome sequence of the filamentous fungus Neurospora crassa reveals potential new targets for antifungals as well as loci implicated in human and plant physiology and disease.

Genomics of Filamentous Fungi

Genomic sequencing is now being extended to the pathogenic yeast, Candida albicans, a hemiascomycete which can grow in unicellular form as well as filamentous form, and Cryptococcus neoformans, a basidiomycete.

Evolutionary genomics of divergence and adaptation within the model fungi Neurospora crassa and Neurospora tetrasperma

The work presented in this dissertation involves the interpretation of patterns of DNA and gene expression variation to infer ways in which evolutionary events and processes have shaped the life

The genome sequence of the model ascomycete fungus Podospora anserina

The features of the P.Anserina genome indicate a highly dynamic evolution since the divergence of P. anserina and N. crassa, leading to the ability of the former to use specific complex carbon sources that match its needs in its natural biotope.

Blueprint of a red mould: Unusual and unexpected findings in the Neurospora genome sequence

Many genes whose presence in Neurospora crassa was unexpected have been revealed in the just published genome sequence (Galagan et al 2003). Among them were two genes that encoded putative homologues

The genome sequence of the rice blast fungus Magnaporthe grisea

The draft sequence of the M. grisea genome is reported, reflecting the clonal nature of this fungus imposed by widespread rice cultivation and analysis of the gene set provides an insight into the adaptations required by a fungus to cause disease.

Fungal genome sequencing: basic biology to biotechnology

  • K. K. Sharma
  • Biology, Engineering
    Critical reviews in biotechnology
  • 2016
The genome sequences provide a first glimpse into the genomic basis of the biological diversity of filamentous fungi and yeast, and provides the road map for basic and applied research.

Neurospora from Natural Populations: Population Genomics Insights into the Life History of a Model Microbial Eukaryote.

The history that brought Neurospora into the era of population genomics is traced, the major contributions of population genomic investigations using neurospora to the understanding of microbial biogeography and speciation are covered, and recent work using population Genomics and genome-wide association mapping is reviewed.

Genome Defense Mechanisms in Neurospora and Associated Specialized Proteins

A variant catalytic subunit of translesion DNA polymerase zeta (Polζ ) has been found to be essential for dominant RIP suppressor phenotype and proteins required for these two processes have been identified through genetic screens.



The Neurospora Compendium: Chromosomal Loci

This compendium provides researchers and students with a concise account of current knowledge about the genes and genome of Neurospora, setting the stage for research that will follow completion of the genome sequence.

Large scale analysis of sequences from Neurospora crassa.

The Neurospora crassa genome: cosmid libraries sorted by chromosome.

A Neurospora crassa cosmid library of 12,000 clones has been created using an improved cosmid vector pLorist6Xh, which contains a bacteriophage lambda origin of replication for low-copy-number replication in bacteria and the hygromycin phosphotransferase marker for direct selection in fungi.

Premeiotic instability of repeated sequences in Neurospora crassa.

  • E. Selker
  • Biology
    Annual review of genetics
  • 1990
Premeiotic intrachromosomal recombination deletes tandem repeats at high frequency in both homothallic and heterothallic filamentous ascomycetes, which potentially stabilizes the gross organization of the genome.

Molecular Biology and Pathogenicity of Mycoplasmas

There is now solid genetic support for the hypothesis that mycoplasmas have evolved as a branch of gram-positive bacteria by a process of reductive evolution and developed various genetic systems providing a highly plastic set of variable surface proteins to evade the host immune system.

The Ashbya gossypii Genome as a Tool for Mapping the Ancient Saccharomyces cerevisiae Genome

The genome of the filamentous ascomycete Ashbya gossypii provided compelling evidence that the evolution of S. cerevisiae included a whole genome duplication orfusion of two related species and showed which of the duplicated genes lost one copy and which retained both copies.

DAB1: a degenerate retrotransposon-like element from Neurospora crassa

Abstract A repeated DNA sequence in the genome of Neurospora crassa has been identified as a family of degenerate retroelements. Retroelements encode protein sequences with clear homology to the

Sequencing and comparison of yeast species to identify genes and regulatory elements

A comparative analysis of the yeast Saccharomyces cerevisiae based on high-quality draft sequences of three related species, which inferred a putative function for most of these motifs, and provided insights into their combinatorial interactions.