The genome sequence of Schizosaccharomyces pombe

  title={The genome sequence of Schizosaccharomyces pombe},
  author={Valerie Wood and Rhian Gwilliam and Marie-Ad{\`e}le Rajandream and M H Lyne and Rachel Lyne and Aengus Stewart and John G. Sgouros and Nigel Peat and Jacqueline Hayles and Stephen Baker and D. Basham and Sharen Bowman and Karen L. Brooks and D. Brown and S. Brown and Tracey Chillingworth and Carol M. Churcher and Matthew David Collins and R. Connor and Ann Cronin and Paul H Davis and Theresa Feltwell and Audrey Fraser and Simone Gentles and Arlette Goble and Nancy Hamlin and David E. Harris and J. Hidalgo and Geoffrey M. Hodgson and Simon Holroyd and T. Hornsby and S. Howarth and Elizabeth J. Huckle and Sarah E. Hunt and Kay Jagels and Keith D. James and L. Jones and M. C. Jones and S. Leather and S. Mcdonald and Joanne Mclean and Paul Mooney and Sharon Moule and Karen L. Mungall and Lee D Murphy and David Niblett and Christy Odell and Karen Oliver and Susan O'neil and Dm Pearson and Michael A. Quail and Ester Rabbinowitsch and Kim Rutherford and Simon R. Rutter and David L. Saunders and Kathy Seeger and Sarah Sharp and Jason Skelton and Mark Simmonds and Rob Squares and Steven Squares and Kim Stevens and K. Taylor and R. G. Taylor and Adrian Tivey and S. Walsh and Tim Warren and Sally Whitehead and John Robert Woodward and G. Volckaert and Rita Aert and Johan Robben and Barbara Grymonprez and I. Weltjens and Els Vanstreels and Michael A. Rieger and M. Sch{\"a}fer and Silke Müller-Auer and C. Gabel and M. Fuchs and C. Fritzc and Elisabeth Holzer and D. Moestl and Helmut Hilbert and Katja Borzym and I. Langer and Alfred Beck and Hans Lehrach and Richard Reinhardt and T. Pohl and Philipp Eger and Wolfgang Zimmermann and Holger Wedler and R. Wambutt and Bénédicte Purnelle and Andr{\'e} Goffeau and {\'E}douard Cadieu and Stéphane Dréano and Stéphanie Gloux and Val{\'e}rie Lelaure and St{\'e}phanie Mottier and Francis Galibert and Stephen J Aves and Zheng Xiang and Cherryl Hunt and Karen Moore and Scott M. Hurst and Mar{\'i}a Lucas and Michel Rochet and C. Gaillardin and V{\'i}ctor A. Tallada and Andr{\'e}s Garz{\'o}n and Guillermo Thode and Rafael R. Daga and L. Cruzado and Juan Jim{\'e}nez and M. S{\'a}nchez and Francisco Escobar del Rey and Javier Benito and A. Dominguez and Jos{\'e} Luis Revuelta and Sergio Moreno and John T. Armstrong and Susan L. Forsburg and L Cerrutti and Todd M. Lowe and W. Richard McCombie and Ian T. Paulsen and Judith A. Potashkin and George V Shpakovski and David Wayne Ussery and Bart Barrell and Paul Nurse},
We have sequenced and annotated the genome of fission yeast (Schizosaccharomyces pombe), which contains the smallest number of protein-coding genes yet recorded for a eukaryote: 4,824. The centromeres are between 35 and 110 kilobases (kb) and contain related repeats including a highly conserved 1.8-kb element. Regions upstream of genes are longer than in budding yeast (Saccharomyces cerevisiae), possibly reflecting more-extended control regions. Some 43% of the genes contain introns, of which… 

Schizosaccharomyces pombe essential genes: a pilot study.

The data suggest that natural selection has preferentially kept the genes required for vital functions in many of the essential eukaryotic genes appeared with the first eUKaryotic cell and have remained conserved in all species.

Population Genomics of the Fission Yeast Schizosaccharomyces pombe

This study sequenced the genomes of 32 worldwide S. pombe strains and examined the pattern of polymorphisms across their genomes, finding that a considerable amount of noncoding DNA is under selective constraint and thus likely to be functional.

Augmented Annotation of the Schizosaccharomyces pombe Genome Reveals Additional Genes Required for Growth and Viability

A systematic reappraisal of the Schizosaccharomyces pombe genome that ignores thresholds is reported, suggesting that all 39 predictions encode functional proteins.

Sequence and analysis of chromosome 2 of Dictyostelium discoideum

A significant number of the genes show higher similarities to genes of vertebrates than to those of other fully sequenced eukaryotes, which strengthens the view that the evolutionary position of D. discoideum is located before the branching of metazoa and fungi but after the divergence of the plant kingdom.

Minimum Genome Factories in Schizosaccharomyces pombe

The genome-reduced strain was less efficient at taking up glucose and some amino acids from the growth media than the parental strain, and showed increased gene expression of the mating pheromone M-factor precursor and NADP-specific glutamate dehydrogenase.

Genome‐wide search of Schizosaccharomyces pombe genes causing overexpression‐mediated cell cycle defects

This work searched for genes causing cell division defects by overexpression in the fission yeast Schizosaccharomyces pombe and identified genes that are involved in a number of cellular processes, which may elucidate novel mechanisms coupling different cellular processes with the control of the cell division.

Comparative analysis of regulatory transcription factors in Schizosaccharomyces pombe and budding yeasts

The budding yeast, Saccharomyces cerevisiae, contains 201 rTfs, which is one of the largest rTF numbers found in yeast species for which genome sequences are available, and this organism is an attractive model for global studies of mechanisms that programme gene expression.

Genomics of Filamentous Fungi

Genomic sequencing is now being extended to the pathogenic yeast, Candida albicans, a hemiascomycete which can grow in unicellular form as well as filamentous form, and Cryptococcus neoformans, a basidiomycete.

Characterization of genome-reduced fission yeast strains

The genome-reduced strain, with a total deletion size of 657.3 kb, showed a decreased ability to uptake glucose and some amino acids in comparison with the parental strain, and showed increased gene expression of the mating pheromone M-factor precursor and the nicotinamide adenine dinucleotide phosphate -specific glutamate dehydrogenase.

An Ancient Yeast for Young Geneticists: A Primer on the Schizosaccharomyces pombe Model System

The fission yeast Schizosaccharomyces pombe appears to have evolved less rapidly than S. cerevisiae so that it retains more characteristics of the common ancient yeast ancestor, causing it to share more features with metazoan cells.



The mitochondrial genome of the fission yeast, Schizosaccharomyces pombe. Sequence of the large-subunit ribosomal RNA gene, comparison of potential secondary structure in fungal mitochondrial large-subunit rRNAs and evolutionary considerations.

An evolutionary evaluation of highly conserved regions of a small set of LSU rRNA sequences suggests that S. pombe mitochondria diverged from a mitochondrial proto-fungal branch earlier than either A. nidulans or S. cerevisiae mitochondria.

The 5S RNA genes of Schizosaccharomyces pombe.

The genomic arrangement and sequences of S. pombe 5S RNA genes are reported here. The 5S gene sequences appear to be dispersed within the genome, and are found independently of other rRNA genes. The

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This is the first complete genome sequence of a plant and provides the foundations for more comprehensive comparison of conserved processes in all eukaryotes, identifying a wide range of plant-specific gene functions and establishing rapid systematic ways to identify genes for crop improvement.

Complete genome structure of the nitrogen-fixing symbiotic bacterium Mesorhizobium loti.

  • T. KanekoY. Nakamura S. Tabata
  • Biology
    DNA research : an international journal for rapid publication of reports on genes and genomes
  • 2000
The complete nucleotide sequence of the genome of a symbiotic bacterium Mesorhizobium loti strain MAFF303099 was determined and suggested that the symbiotic island as well as the plasmids originated and were transmitted from other genetic systems.

Molecular evidence for an ancient duplication of the entire yeast genome

A model is proposed in which this species is a degenerate tetraploid resulting from a whole-genome duplication that occurred after the divergence of Saccharomyces from Kluyveromyces, and protein pairs derived from this duplication event make up 13% of all yeast proteins.

Subtelomeric sequence from the right arm of Schizosaccharomyces pombe chromosome I contains seven permease genes

The sequence has been determined of 80 888 bp of contiguous subtelomeric DNA, including the isp5 gene, from the right arm of chromosome I of Schizosaccharomyces pombe; 27 open reading frames (ORFs)

The 5.8S RNA gene sequence and the ribosomal repeat of Schizosaccharomyces pombe.

Analysis of isolated total cellular 5.8S RNA revealed the presence of eight species of 5.7S RNA, differing in the number of nucleotides at the 5'-end, and the sequence of the eight 5.4S RNA species appears to be identical and is the same sequence as coded for by the 5.