The genome sequence of Drosophila melanogaster.

  title={The genome sequence of Drosophila melanogaster.},
  author={Mark D. Adams and Susan E. Celniker and Robert A. Holt and Cheryl A. Evans and J. D. Gocayne and Peter G. Amanatides and Steven E. Scherer and P W Li and Roger A. Hoskins and Richard F. Galle and Reed A. George and Suzanna E. Lewis and Stephen Richards and Michael Ashburner and Shirley Henderson and Granger G. Sutton and Jennifer R. Wortman and Mark Yandell and Q. Zhang and L Chen and Rhonda Brandon and Yu Hui Rogers and R G Blazej and Mark A. Champe and Barret D. Pfeiffer and Kenneth H. Wan and C Doyle and Ellis G. Baxter and Gregg A. Helt and Catherine R. Nelson and G. G{\'a}bor and Josep F. Abril and Aguedo F. Agbayani and Huijin An and Cynthia Andrews-Pfannkoch and Danita Baldwin and Richard Ballew and Anand Basu and J. W. Baxendale and Leyla Bayraktaroglu and Ellen M. Beasley and Karen Beeson and Panayiotis V. Benos and Benjamin Paul Berman and Deepak Bhandari and Slava Bolshakov and D. Borkova and Michael R. Botchan and John Bouck and Peter B Brokstein and Philippe Brottier and Kenneth C. Burtis and Dana Busam and H Butler and {\'E}douard Cadieu and Angela Center and Ishwar Chandra and J. Michael Cherry and Simon E. Cawley and Carl Dahlke and Lionel B. Davenport and Polly Davies and Beatriz de Pablos and Arthur L. Delcher and Z Q Deng and Anne Deslattes Mays and Ian M. Dew and Susanne Malchau Dietz and Kristina L. Dodson and Lisa Doup and M Downes and Shannon Dugan-Rocha and Boris C. Dunkov and Patrick Dunn and K James Durbin and Carlos Evangelista and Conchita Ferraz and Steve Ferriera and Wolfgang Fleischmann and Carl Fosler and Andrei E. Gabrielian and Naveen Garg and William M. Gelbart and Kenneth Glasser and Anna Glodek and Fangcheng Gong and James H. Gorrell and Zhiping Gu and Ping Guan and Michael Harris and Nomi L. Harris and D Harvey and Thomas J. Heiman and J. Rosete Hernandez and Jarrett T. Houck and Damon Hostin and Kathryn A. Houston and T. Howland and Ming Hui Wei and Chinyere Ibegwam and Mena Jalali and Francis Kalush and Gary H. Karpen and Zhang Ke and James A. Kennison and Karen A. Ketchum and Bruce E. Kimmel and Chinnappa D. Kodira and Cheryl L. Kraft and Saul A. Kravitz and David Kulp and Zhongwu Lai and Paul Lasko and Yiding Lei and Alexander Levitsky and J. Li and Z Li and Y. T. Liang and X Lin and X. Liu and Bill Mattei and Tina C. McIntosh and Michael P. McLeod and Daniel K. McPherson and Gennady V. Merkulov and Natalia Milshina and Clark M. Mobarry and Jordan L. Morris and A Moshrefi and Stephen M. Mount and Mee C. Moy and Brian Murphy and Lee D Murphy and D. Muzny and David Lee Nelson and Donnie R. Nelson and Keith A. Nelson and K. J. Nixon and Deborah R. Nusskern and Joanne Pacleb and Michael J. Palazzolo and Gary S. Pittman and Sue Pan and Jill L. Pollard and Vinita Puri and Martin G. Reese and Knut Reinert and Karin A. Remington and Robert D. C. Saunders and F Scheeler and H Shen and Bixiong Chris Shue and Inga Siden-Kiamos and Michael Simpson and Marian Skupski and T Smith and Eugene G. Spier and Allan C. Spradling and Mark Stapleton and Renee Strong and E T Sun and Robert R. Svirskas and Cy Tector and Rudolf Turner and Eli Venter and A H Wang and X Wang and Z. Y. Wang and David A. Wassarman and George M. Weinstock and Jean Weissenbach and S. Williams and WoodageT and Kim C. Worley and D Wu and S. Yang and Qiang Yao and J. Ye and Rufang Yeh and Jayshree Zaveri and Ming Zhan and G. X. Zhang and Q Zhao and L. Zheng and X. H. Zheng and Fei Zhong and Wenyan Zhong and X. R. Zhou and S. Zhu and X. Zhu and H. O. Smith and Richard A. Gibbs and Eugene Wimberly Myers and Gerald M. Rubin and J. Craig Venter},
  volume={287 5461},
The fly Drosophila melanogaster is one of the most intensively studied organisms in biology and serves as a model system for the investigation of many developmental and cellular processes common to higher eukaryotes, including humans. We have determined the nucleotide sequence of nearly all of the approximately 120-megabase euchromatic portion of the Drosophila genome using a whole-genome shotgun sequencing strategy supported by extensive clone-based sequence and a high-quality bacterial… 

The Drosophila melanogaster genome.

The current state of the Drosophila melanogaster genome sequence and its structural annotation is reviewed and some promising approaches that are being taken to achieve an initial functional annotation are summarized.

Drosophila melanogaster: a case study of a model genomic sequence and its consequences.

The impact of the growing number of genome sequences now available in the genus on current Drosophila research, and some of the biological questions that these resources will enable to be solved in the future are discussed.

The Drosophila genome.

  • S. Celniker
  • Biology
    Current opinion in genetics & development
  • 2000

The fruits of the fly genome project.

The Release 6 reference sequence of the Drosophila melanogaster genome

An improved reference sequence of the single-copy and middle-repetitive regions of the genome is reported, produced using cytogenetic mapping to mitotic and polytene chromosomes, clone-based finishing and BAC fingerprint verification, ordering of scaffolds by alignment to cDNA sequences, incorporation of other map and sequence data, and validation by whole-genome optical restriction mapping.

Systematic gene targeting on the X chromosome of Drosophila melanogaster

Single UAS sequence-bearing P-element insertions throughout the X chromosome are described, which allows one to express the tagged genes under control of tissue/organ-directed GAL4 activity and provides a tool to generate chromosomal deletions.

The Drosophila genome(s)

It is considered how the polytene chromosomes may provide a system to move from the linear DNA sequence to the challenging area of chromatin organization and chromosome structure.

The 19 Genomes of Drosophila: A BAC Library Resource for Genus-Wide and Genome-Scale Comparative Evolutionary Research

The first synthesis of a comprehensive set of bacterial artificial chromosome resources for 19 Drosophila species from all three subgenera is reported, demonstrating the utility of this BAC resource for generating physical maps of targeted loci, refining draft sequence assemblies and identifying potential genomic rearrangements across the phylogeny.

Interpreting the regulatory genome: the genomics of transcription factor function in Drosophila melanogaster.

With an estimated 700 DNA-binding proteins in the Drosophila genome, it will be many years before each potential sequence-specific TF is studied in detail, yet the last decade of functional genomics research has already impacted the view of gene regulatory networks and TF DNA recognition.

Comparative genomics of the eukaryotes.

The fly has orthologs to 177 of the 289 human disease genes examined and provides the foundation for rapid analysis of some of the basic processes involved in human disease.



Comparative genomics of the eukaryotes.

The fly has orthologs to 177 of the 289 human disease genes examined and provides the foundation for rapid analysis of some of the basic processes involved in human disease.

Towards a Drosophila genome map.

The whole genome assembly of Drosophila

The quality of a whole-genome assembly of Drosophila melanogaster and the nature of the computer algorithms that accomplished it are reported on and should be of substantial value to the scientific community.

A brief history of Drosophila's contributions to genome research.

The sequence of the Drosophila melanogaster genome presented in this issue of Science is the latest milestone in nine decades of research on this organism. Genetic and physical mapping, whole-genome

A BAC-based physical map of the major autosomes of Drosophila melanogaster.

A bacterial artificial chromosome (BAC)-based physical map of chromosomes 2 and 3 of Drosophila melanogaster, which constitute 81% of the genome, is constructed to produce a map spanning the euchromatin.

An exploration of the sequence of a 2.9-Mb region of the genome of Drosophila melanogaster: the Adh region.

This is the first large-scale analysis of the genome of D. melanogaster at the sequence level, and has allowed us to develop and test methods that will be needed to interpret the complete sequence of the genomes of this species.

Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana

The sequence of chromosome 2 from the Columbia ecotype is reported in two gap-free assemblies (contigs) of 3.6 and 16 megabases, which represents the longest published stretch of uninterrupted DNA sequence assembled from any organism to date.

The Berkeley Drosophila Genome Project gene disruption project: Single P-element insertions mutating 25% of vital Drosophila genes.

The results show that Drosophila genes have a wide range of sensitivity to inactivation by P elements, and provide a rationale for greatly expanding the BDGP primary collection based entirely on insertion site sequencing, and predict that this approach can bring >85% of all Dosophila open reading frames under experimental control.