The genome organization of the broad bean necrosis virus (BBNV)

  title={The genome organization of the broad bean necrosis virus (BBNV)},
  author={X Y Lu and S. Yamamoto and M. Tanaka and Tadaaki Hibi and Shigetou Namba},
  journal={Archives of Virology},
SummaryThe genome of the broad bean necrosis virus Oita-isolate (BBNV-O) [RNAff1 (6.0 kb), RNAff2 (2.8 kb) and RNAff3 (2.4 kb)] was cloned and sequenced. Computer analysis indicates that methyltransferase, helicase and RNA-dependent RNA polymerase (RdRp) motifs are present in RNAff1. The viral capsid protein (CP) cistron is located at the 5′ terminal end of RNAff2 and the Mr of CP (20 K) is close to that determined by SDS-PAGE analysis. An ochre codon (UAA) in the CP cistron is thought to be… 
The readthrough region of Potato mop-top virus (PMTV) coat protein encoding RNA, the second largest RNA of PMTV genome, undergoes structural changes in naturally infected and experimentally inoculated plants
The complete sequence of “the coat protein (CP) encoding RNA” of an isolate of PMTV obtained from the field in Sweden is reported, and considerable variability and occurrence of deletion variants of RT in the field isolates of PM television are revealed.
Molecular characterization of Potato mop-top virus isolates from China and Canada and development of RT-PCR differentiation of two sequence variant groups
The complete genome comprising three genomic RNAs of three Canadian and two Chinese isolates of Potato mop-top virus were sequenced and analysed, finding each of RNA1, RNA2 and RNA3 could be divided into at least two groups.
Composition of alphavirus-like replication complexes: involvement of virus and host encoded proteins
Current knowledge on the composition of the RdRp’s of alpha-like viruses from supergroup III is reviewed and the strategy for the expression of viral replicase proteins, their stoichiometry in the enzyme complex, their mutual interactions and their possible functions in RNA synthesis are discussed.
Structure and variability of the 3′ end of RNA 3 of Beet soil-borne pomovirus– a virus with uncertain pathogenic effects
Summary. PCR products representing c. 550 3′ terminal bases of Beet soil-borne pomovirus (BSBV) RNA 3 were compared for sourcesof this virus from all major sugarbeet-growing areas in Germany. In
Detection of potato mop-top virus in potato tubers and sprouts: combinations of RNA2 and RNA3 variants and incidence of symptomless infections.
Sequencing and restriction fragment length polymorphism analysis of polymerase chain reaction amplicons indicated that the majority of PMTV isolates infecting tubers comprise restrictotypes RNA2-II andRNA3-B, and no specific combination of the types of RNA2 and RNA3 was associated with spraing-expressing or symptomless tubers.
Fungally-transmitted Rod-shaped Plant Viruses: Biology, Transmission and Molecular Pathology
The association of lack of transmissibility ofPMTV-T with-in apparent deletion of sequence in RT, relative to RT of transmissible isolate PMTV-S suggests that the RT domain, encoded by PMTV -S RNA 3 contains determinants that play an important role in the acquisition and transmission of PMTV by S. subterranea.
Genome Organization in RNA Viruses
Four distinct supergroups of plus-sense RNA plant viruses are now recognized, including the `sobemo-like“ and ”carmo- like“ supergroups.
RNA silencing machinery contributes to inability of BSBV to establish infection in Nicotiana benthamiana: evidence from characterization of agroinfectious clones of Beet soil-borne virus.
The entire viral cycle was reproduced, validating the constructed agroinfectious clones as efficient inoculation tools, paving the way for further studies on BSBV and its related pathosystem.
Transient coexpression of individual genes encoded by the triple gene block of potato mop-top virus reveals requirements for TGBp1 trafficking.
Transient expression of Potato mop-top virus was used as a model to reconstruct interactions between TGB proteins and mutagenesis suggested that the NTPase and helicase activities of TGBp1 were not required for its transport to intermediate bodies, but these activities were essential for the protein association with cell wall-embedded punctate bodies and translocation of T GBpl to neighboring cells.
Ecology and epidemiology of benyviruses and plasmodiophorid vectors.
  • C. Rush
  • Biology
    Annual review of phytopathology
  • 2003
Both Beet necrotic yellow vein virus and Beet soilborne mosaic virus are widespread and frequently found in the same field, infecting the same beet plant, and the implications of this close proximity are uncertain.


Nucleotide Sequence of Beet Necrotic Yellow Vein Virus RNA-2
The complete nucleotide sequence of beet necrotic yellow vein virus (BNYVV) RNA-2 has been determined from a study of cloned cDNA and evidence is presented that the 42K polypeptide is expressed, probably from a subgenomic messenger RNA.
Complete nucleotide sequence and organization of the bipartite RNA genome of soil-borne wheat mosaic virus.
Differences in the 3' end structure and in the cell-to-cell movement protein, and the distant phylogeny of the RNA replicase genes of SBWMV and beet necrotic yellow vein virus, suggest that the furoviruses should be divided into at least two groups.
Evidence that the 75K readthrough protein of beet necrotic yellow vein virus RNA-2 is essential for transmission by the fungus Polymyxa betae.
Results indicate that the 75K readthrough protein encoded by RNA-2 is essential for the transmission of BNYVV by P. betae.
The nucleotide sequence of potato mop-top virus RNA 2: a novel type of genome organization for a furovirus.
The gene arrangement in PMTV is therefore novel for a furovirus, having a tripartite genome and sequence similarity to the triple-gene-block proteins of other viruses, particularly barley stripe mosaic hordeivirus.
Nucleotide sequence and genetic organization of peanut clump virus RNA 2 and partial characterization of deleted forms.
The complete nucleotide sequence of peanut clump virus RNA 2 has been determined by characterization of cloned cDNA and by direct RNA sequencing and partial characterization of two such isolates reveals that their RNA 2's have undergone deletions within ORF 2.
Production and Pathogenicity of Isolates of Beet Necrotic Yellow Vein Virus with Different Numbers of RNA Components
Summary Ten Japanese field isolates of beet necrotic yellow vein virus (BNYVV) were transmitted to Tetragonia expansa by inoculation with sap from rootlets of sugar-beet seedlings, to which the virus
Evolution and taxonomy of positive-strand RNA viruses: implications of comparative analysis of amino acid sequences.
It is hypothesized that all positive-strand RNA viruses and some related double-stranded RNA viruses could have evolved from a common ancestor virus that contained genes for RNA-dependent RNA polymerase, a chymotrypsin-related protease that also functioned as the capsid protein, and possibly an RNA helicase.