The genetics and genomics of the silkworm, Bombyx mori.

  title={The genetics and genomics of the silkworm, Bombyx mori.},
  author={Marian R Goldsmith and T. Shimada and Hiroaki Abe},
  journal={Annual review of entomology},
We review progress in applying molecular genetic and genomic technologies to studies in the domesticated silkworm, Bombyx mori, highlighting its use as a model for Lepidoptera, and in sericulture and biotechnology. Dense molecular linkage maps are being integrated with classical linkage maps for positional cloning and marker-assisted selection. Classical mutations have been identified by a candidate gene approach. Cytogenetic and sequence analyses show that the W chromosome is composed largely… 

Genetic mapping of Z chromosome and identification of W chromosome-specific markers in the silkworm, Bombyx mori

A genetic map of randomly amplified polymorphic DNA fragments (RAPD), simple sequence repeats (SSR), and fluorescent intersimple sequence repeat PCR (FISSR) markers for the Z chromosome is constructed using a backcross mapping population to identify and map sex-linked traits in the silkworm.

A Second-Generation Integrated Map of the Silkworm Reveals Synteny and Conserved Gene Order Between Lepidopteran Insects

A second-generation linkage map was constructed for the silkworm, Bombyx mori, and found significant synteny and conserved gene order between B. mori and a nymphalid butterfly, Heliconius melpomene, in four linkage groups (LGs), strongly suggesting that using B.mori as a reference for comparative genomics in Lepidotera is highly feasible.

Highly Efficient Targeted Gene Disruption in the Silkworm, Bombyx mori, Using Genome Editing Tools

This chapter summarizes recent advances in genome editing techniques in B. mori and proposes guidelines for experimental design and strategy for successful gene knockout experiments using this species.

Molecular Marker-Assisted Selection Breeding in Silkworm, Bombyx mori

To keep up the pace with the technology, it has become necessary for the breeder to be familiar with the advancement in the field of DNA-marker technology and to turn into biotechnologists.

Horizontal gene transfer in silkworm, Bombyx mori

The whole genome of B. mori is compared with those of 382 prokaryotic and eukaryotic species to investigate the potential horizontal gene transfer (HGT) genes and functional analysis revealed that several HGT candidate genes have added important characters, such as resistance to pathogen, to B.mori.

Genome editing in Bombyx mori: New opportunities for silkworm functional genomics and the sericulture industry

The progress and potential of genome editing in silkworm and its applications in functional genomic studies for generating novel genetic materials are summarized.

Recent progress in genome engineering techniques in the silkworm, Bombyx mori

TALENs can now be used as a standard tool for gene targeting studies, even when mutant phenotypes are unknown, and will greatly increase the usefulness of Bombyx as a model for lepidopteran insects, the major agricultural pests, and lead to sophisticated breeding of Bomberx for use in sericulture and biotechnology.

FLP Recombinase-Mediated Site-Specific Recombination in Silkworm, Bombyx mori

It is concluded that the FLP/FRT system is a useful tool for genome manipulation in the silkworm and provides a useful reference for the establishment of genome manipulation technologies in other lepidopteran species.

Linkage Analysis of Bombyx mori Sex-controlled Melanism(sml) Mutation Using Morphological and Molecular Markers

Results showed that sml gene was linked with molecular markers on the 17th linkage group(corresponding to chromosome 17), but was independent with morphological markers or molecular marker on the other linkage groups,Therefore, sml mutational gene is located on the17th chromosome of silkworm.

Genome Survey and Mitochondrial Genome Analysis of Wild Silkworm, Bombyx mandarina

This study supports the contention that domestic of silkworm descended from the northern of China, and implied that the Qin-ba mountain area could be one of the original regions of domestic silkworm.



Silkworm genomics - progress and prospects

BAC libraries have been constructed and are being used to make large-scale physical maps, with markers based on ESTs as framework anchors, which provides a foundation for identification of gene function, gene and chromosome evolution, and comparative genomics.

Recent advances in molecular genetics of the silk moth, Bombyx mori

The domesticated silk moth, Bombyx mori, the lepidopteran molecular model and an important economic insect is emerging as an ideal molecular genetic resource for solving a broad range of biological

A genetic linkage map for the domesticated silkworm, Bombyx mori , based on restriction fragment length polymorphisms

We present data for the initial construction of a molecular linkage map for the domesticated silkworm, Bombyx mori , based on 52 progeny from an F2 cross from a pair mating of inbred strains p50 and

Construction and characterization of bacterial artificial chromosome libraries from the silkworm, Bombyx mori

Results show that it is possible to construct contigs and analyze chromosome organization using these libraries, and revealed novel information about the chromosomal organization of the sericin-1 and DH-PBAN genes, which were localized within a 22-kb interval and are divergently oriented.

Retrotransposable elements on the W chromosome of the silkworm, Bombyx mori

It is found that many long terminal repeat (LTR) and non-LTR retrotransposons, retroposons, DNA transposons and their derivatives, have accumulated on the W chromosome as strata.

Germline transformation of the silkworm Bombyx mori L. using a piggyBac transposon-derived vector

The presence of the inverted terminal repeats of piggyBac and the characteristic TTAA sequence at the borders of all the analyzed inserts confirmed that transformation resulted from precise transposition events.

Gene targeting in the silkworm by use of a baculovirus.

Analysis showed that the chimeric gene had integrated into the L-chain gene on the genome by homologous recombination and was stably transmitted through generations.

Heat-inducible transgenic expression in the silkmoth Bombyx mori

Germline transformation with new transposon vectors now enables causal tests of gene function via ectopic protein expression or RNA interference in non-drosophilid insects and proves heat induction of the transgenic BmFtz-F1 was proven at both the mRNA and protein levels.

A dense genetic map of the silkworm, Bombyx mori, covering all chromosomes based on 1018 molecular markers.

A dense linkage map was constructed for the silkworm, Bombyx mori, containing 1018 genetic markers on all 27 autosomes and the Z chromosome that is the first linkage map in insects having a large number of chromosomes that covers all chromosomes without any gaps.

Identification and characterisation of a silkworm ABC transporter gene homologous to Drosophila white

ABC transporter genes homologous to the Drosophilawhite gene were isolated from the silkworm to understand the pigment precursor transporters involved and characterised this gene further, finding that Bmwh3 is a single-copy gene.