The generality of self-splicing RNA: Relationship to nuclear mRNA splicing

  title={The generality of self-splicing RNA: Relationship to nuclear mRNA splicing},
  author={Thomas R. Cech},
  • T. Cech
  • Published 31 January 1986
  • Biology
  • Cell

A ribozyme selected from variants of U6 snRNA promotes 2',5'-branch formation.

The finding that lariat formation can be accomplished by a small RNA with sequences related to U6 snRNA indicates that the RNA available in the spliceosome may be involved in RNA-catalyzed branch formation.

Contributions to the study of the architecture and evolution of ribozymes

The pk-turn, a new RNA motif related to k-turns that allow for the formation of a bend of 60° between stems P16 and P17 from the bacterial RNaseP, is identified.

Crystal structure of a group I intron splicing intermediate.

Models of the pre-first and pre-second steps of intron splicing are proposed with full-sized tRNA exons, suggesting that the tRNA undergoes substantial angular motion relative to the intron between the two steps of splicing.

The NADH-dehydrogenase subunit 5 gene in Oenothera mitochondria contains two introns and is co-transcribed with the 5 S rRNA gene

The gene encoding subunit 5 of the NADH dehydrogenase complex (ND 5) has been identified in Oenothera mitochondria from a cDNA clone andcision of the two introns appears to proceed slowly in vivo since the steady state mitochondrial RNA contains significant proportions of unprocessed precursor molecules.

An overview of RNA splicing and functioning of splicing factors in land plant chloroplasts

Recent progress on the splicing factors in land plant chloroplasts is summarized and their possible roles in chloroplast RNA splicing are discussed based on previous studies.

Group II Intron-Encoded Proteins (IEPs/Maturases) as Key Regulators of Nad1 Expression and Complex I Biogenesis in Land Plant Mitochondria

The following review provides an update on the non-canonical MAT factors in angiosperm mitochondria and summarize the current knowledge of their essential roles in regulating Nad1 expression and complex I (CI) biogenesis during embryogenesis and early plant life.

Mechanisms of catalytic RNA molecules.

The main features of the mechanisms of various types of ribozymes that can function with or without the help of proteins to perform their biological functions are described.