The gene-gun approach for transfection and labeling of cells in brain slices.

@article{Dunaevsky2013TheGA,
  title={The gene-gun approach for transfection and labeling of cells in brain slices.},
  author={A Dunaevsky},
  journal={Methods in molecular biology},
  year={2013},
  volume={1018},
  pages={111-8}
}
Biolistic transfection and diolistic labeling are techniques in which subcellular-sized particles, coated with DNA and lipophilic dyes, respectively, are propelled into cells. The gene-gun approach is particularly applicable for use on ex vivo organized tissue such as brain slices, where cells are not accessible for transfection with methods used in… CONTINUE READING