The gene controlling marijuana psychoactivity: molecular cloning and heterologous expression of Delta1-tetrahydrocannabinolic acid synthase from Cannabis sativa L.

@article{Sirikantaramas2004TheGC,
  title={The gene controlling marijuana psychoactivity: molecular cloning and heterologous expression of Delta1-tetrahydrocannabinolic acid synthase from Cannabis sativa L.},
  author={Supaart Sirikantaramas and Satoshi Morimoto and Yoshinari Shoyama and Yu Ishikawa and Yoshiko Wada and Yukihiro Shoyama and Futoshi Taura},
  journal={The Journal of biological chemistry},
  year={2004},
  volume={279 38},
  pages={39767-74}
}
Delta(1)-tetrahydrocannabinolic acid (THCA) synthase is the enzyme that catalyzes oxidative cyclization of cannabigerolic acid into THCA, the precursor of Delta(1)-tetrahydrocannabinol. We cloned a novel cDNA (GenBank trade mark accession number AB057805) encoding THCA synthase by reverse transcription and polymerase chain reactions from rapidly expanding leaves of Cannabis sativa. This gene consists of a 1635-nucleotide open reading frame, encoding a 545-amino acid polypeptide of which the… CONTINUE READING
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