The gelatin sponge–chorioallantoic membrane assay

Abstract

Here we present a method for the quantification of angiogenesis and antiangiogenesis in the chick embryo chorioallantoic membrane (CAM) based on the implantation of a gelatin sponge on the top of the growing CAM on day 8 of development. After implantation, the sponge is treated with a stimulator of blood vessel formation in the absence or presence of an… (More)
DOI: 10.1038/nprot.2006.13

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