The gelatin-binding site of the second type-II domain of gelatinase A/MMP-2.

@article{Tordai1999TheGS,
  title={The gelatin-binding site of the second type-II domain of gelatinase A/MMP-2.},
  author={Hedvig Tordai and L{\'a}szl{\'o} Patthy},
  journal={European journal of biochemistry},
  year={1999},
  volume={259 1-2},
  pages={513-8}
}
We have shown previously that all three fibronectin type-II modules of gelatinase A contribute to its gelatin affinity. In the present work the second type-II module was subjected to site-directed mutagenesis in order to localize its gelatin-binding site. The functional integrity of mutant proteins was assessed by their affinity for gelatin using gelatin-Sepharose affinity chromatography. The structural integrity of the mutant proteins, i.e. their resistance to thermal and chaotropic agent… CONTINUE READING

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