The fur homologue in Borrelia burgdorferi.

  title={The fur homologue in Borrelia burgdorferi.},
  author={Laura I. Katona and Rafal Tokarz and Christopher J Kuhlow and Jordi Benach and Jorge L. Benach},
  journal={Journal of bacteriology},
  volume={186 19},
Borrelia burgdorferi contains a gene that codes for a Fur homologue. The function of this Fur protein is unknown; however, spirochetes grown at 23 or 35 degrees C expressed fur as determined by reverse transcriptase PCR. The fur gene (BB0647) was cloned and overexpressed as a His-Fur fusion protein in Escherichia coli. The fusion protein was purified by zinc-chelate chromatography, and the N-terminal His tag was removed to generate recombinant Fur for use in mobility shift studies. Fur bound… CONTINUE READING


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