The functional role of basic patch, a structural element of Escherichia coli transcript cleavage factors GreA and GreB.

@article{Kulish2000TheFR,
  title={The functional role of basic patch, a structural element of Escherichia coli transcript cleavage factors GreA and GreB.},
  author={D Kulish and J Lee and Ivan B. Lomakin and Beatrycze Nowicka and Aditi Das and Seth A Darst and K Normet and Sergei Borukhov},
  journal={The Journal of biological chemistry},
  year={2000},
  volume={275 17},
  pages={12789-98}
}
The transcript cleavage factors GreA and GreB of Escherichia coli are involved in the regulation of transcription elongation. The surface charge distribution analysis of their three-dimensional structures revealed that the N-terminal domains of GreA and GreB contain a small and large basic "patch," respectively. To elucidate the functional role of basic patch, mutant Gre proteins were engineered in which the size and charge distribution of basic patch were modified and characterized… CONTINUE READING