The fragile X mental retardation protein interacts with U-rich RNAs in a yeast three-hybrid system.

@article{Dolzhanskaya2003TheFX,
  title={The fragile X mental retardation protein interacts with U-rich RNAs in a yeast three-hybrid system.},
  author={Natalia Dolzhanskaya and Ying Ju Sung and James Conti and Julia R. Currie and Robert B. Denman},
  journal={Biochemical and biophysical research communications},
  year={2003},
  volume={305 2},
  pages={434-41}
}
We recently identified several ESTs that bind to the fragile X mental retardation protein (FMRP) in vitro. To determine whether they interacted in vivo we performed three-hybrid screens in a Saccharomyces cerevisiae histidine auxotroph. We demonstrate that two of the ESTs support growth on histidine and transduce beta-galactosidase activity when co-expressed with FMRP under selective growth conditions. In contrast, the iron response element (IRE) RNA does not. Likewise, the ESTs do not support… CONTINUE READING

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