The fractionation of high-molecular-weight ribonucleic acid by polyacrylamide-gel electrophoresis.

@article{Loening1967TheFO,
  title={The fractionation of high-molecular-weight ribonucleic acid by polyacrylamide-gel electrophoresis.},
  author={U. Loening},
  journal={The Biochemical journal},
  year={1967},
  volume={102 1},
  pages={
          251-7
        }
}
  • U. Loening
  • Published 1967
  • Chemistry, Medicine
  • The Biochemical journal
1. Gels were prepared with recrystallized acrylamide and bisacrylamide. Electrophoresis was in tris-sodium acetate-EDTA buffer for 0.5 to 3hr. Gels were scanned at 280 or 265mmu. Techniques are described for slicing and radioactive counting. 2. The mobility of RNA was inversely related to the sedimentation coefficient and varied with gel concentration. Electrophoresis in 2.2-2.6% gels gives a fractionation similar to density-gradient centrifugation. It shows the two ribosomal RNA components… Expand
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