The fractionation of high-molecular-weight ribonucleic acid by polyacrylamide-gel electrophoresis.

  title={The fractionation of high-molecular-weight ribonucleic acid by polyacrylamide-gel electrophoresis.},
  author={Ulrich E. Loening},
  journal={The Biochemical journal},
  volume={102 1},
  • U. Loening
  • Published 1967
  • Chemistry, Medicine
  • The Biochemical journal
1. Gels were prepared with recrystallized acrylamide and bisacrylamide. Electrophoresis was in tris-sodium acetate-EDTA buffer for 0.5 to 3hr. Gels were scanned at 280 or 265mmu. Techniques are described for slicing and radioactive counting. 2. The mobility of RNA was inversely related to the sedimentation coefficient and varied with gel concentration. Electrophoresis in 2.2-2.6% gels gives a fractionation similar to density-gradient centrifugation. It shows the two ribosomal RNA components… Expand
Fractionation of Ribonucleic Acids by ‘Sephadex’ Agarose Gel Electrophoresis
Loening used the polyacrylamide gel electrophoresis recommended by Richards et al.3 and was able to obtain a very good separation of the two ribosomal fractions and transfer RNA in 2.2 per cent and 2.6 per cent gels. Expand
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Polyacrylamide gels containing 15% acrylamides provide a molecular sieve of unique resolving power for electrophoretic separation of low molecular weight RNA on the basis of size, and the exact relation between mobility and sedimentation rates is reported. Expand
Simple agar--urea-gel electrophoretic fractionation of high molecular weight ribonucleic acids.
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The method has been applied to the complete separation of RNA fractions obtained after a preliminary gel electrophoresis of partial enzymic digests of 32P-labeled bacteriophage RNA. Expand
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The polymerization time of the composite gel is extended and the gel has increased mechanical stability, and their suitability for staining and absorbance scanning is not altered. Expand
Microelectrophoretic Technique for Fractionation of RNA
GEL electrophoresis separates RNA fractions with higher resolution than the commonly used techniques of column ehromatography and sucrose density gradient centrifugation, and is described as a procedure which permits fractionation of native RNA in amounts of 10−9 to 10−10 g. Expand
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A technique is described for the preparation of 2.5% to 12% polyacrylamide gradient gels and its use in the separation by gel electrophoresis of total RNA preparations, which can be separated on a single gel with good resolution. Expand
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  • H. Birnboim
  • Chemistry, Medicine
  • Biochimica et biophysica acta
  • 1972
The entire procedure is relatively rapid; the desalting step requires 3 h and electrophoresis about 2 h, and nucleotides are readily recovered for further analysis or for determination of radioactivity. Expand
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A method is described which permits the purification of proteins in quantities necessary for physicochemical characterization by electrophoresis in a number of sodium dodecyl sulfate (SDS)-containing polyacrylamide disc gels. Expand