The foundation of two distinct cell lineages within the mouse morula

  title={The foundation of two distinct cell lineages within the mouse morula},
  author={Martin H. Johnson and Carol Ann Ziomek},

Properties of polar and apolar cells from the 16-cell mouse morula

The surface properties of newly formed, isolated 1/16 mouse blastomeres have been analyzed over the 10–12 h period prior to their division to 2/32 cells and the results are discussed in the context of the normal fate and potential of each cell type within the morula.

The influence of cell contact on the division of mouse 8-cell blastomeres.

It is suggested that cell interactions immediately prior to and during division do not influence strongly the orientation and position of the division plane, and interactions between the cells within an intact early 8-cell embryo do influence the type of progeny generated at division to the 16-cell stage.

Oriented cell divisions asymmetrically segregate aPKC and generate cell fate diversity in the early Xenopus embryo

It is shown that oriented cell divisions generate the superficial and deep cells and establish cell fate diversity between them in Xenopus and only the progeny of the cells that inherit the apical membrane turn on the bHLH gene, ESR6e.

Origin and formation of the first two distinct cell types of the inner cell mass in the mouse embryo

A model in which the timing of cell internalization, cell position, and cell sorting combine to determine distinct lineages of the preimplantation mouse embryo is proposed.

Cytocortical organization during natural and prolonged mitosis of mouse 8-cell blastomeres.

In mitotic cells at all stages from early metaphase to immediately postcytokinesis microvilli were found to be present over more of the cell surface, suggesting a heterogeneity of cytocortical organization may still exist within the arrested mitotic cell.

The developmental potential of mouse 16-cell blastomeres.

Postimplantation development of blastocysts derived from aggregates of outer or inner cells, after transfer to pseudopregnant recipients, was normal and comparable to zona-intact control embryos.

Cell Polarity in the Preimplantation Mouse Embryo

Attempts to elucidate the mechanisms by which these two distinct cell types diverge from a common pathway during the first 3 days of preimplantation development have yielded not only clues as to possible differentiative signals operating in development, but also detailed structural information on the morphology and properties of cells from the early embryo.



Distribution of microvilli on dissociated blastomeres from mouse embryos: evidence for surface polarization at compaction.

When late 8-cell embryos were disaggregated to single cells, and these sorted by pattern of fluorescent-ligand binding, each of the four patterns of staining related consistently to a characteristic distribution of microvilli as viewed by scanning electron microscopy.

Development of blastomeres of mouse eggs isolated at the 4- and 8-cell stage.

Investigations by Seidel and Tarkowski have shown that although the majority of 1/2 blastomeres can regulate and develop into smaller but otherwise normal blastocysts, som e of them give rise to purely trophoblastic vesicles devoid of the inner cell mass.

Role of the blastocoele microenvironment in early mouse embryo differentiation

Results are reported, obtained by injecting donor cells into host blastocysts, showing that totipotent cells exposed only to blastocoele fluid differentiate into morphologically normal blastocyts, whereas those in contact with the blastocyst's inner surface do not.

The effect of spatial arrangement on cell determination during mouse development.

It was concluded that at these stages cell position could determine the development of blastomeres and the segregation of morphogenetic factors at the 4- and 8-cell stages of mouse development.

Formation and consequences of cell patterns in preimplantation mouse development.

The results suggest that the morphogenetic movements of these early embryonic cells are principally governed by continuous cell interactions after fertilization.

Changes in cell surface and cortical cytoplasmic organization during early embryogenesis in the preimplantation mouse embryo

Observations suggest that morphogenesis involves the activation of a developmental program which coordinately controls cortical cytoplasmic and cell surface organization in embryogenesis.

Investigation of the determinative state of the mouse inner cell mass. II. The fate of isolated inner cell masses transferred to the oviduct.

  • J. Rossant
  • Biology
    Journal of embryology and experimental morphology
  • 1975
Although no trophoblast was formed, 3 1/2-day ICMs formed an outer endoderm layer after 1 day in the oviduct, as judged by light and electron microscopical evidence, and it is suggested that cell position may be important inendoderm differentiation.

Cytoplasmic polarity develops at compaction in rat and mouse embryos.

  • W. Reeve
  • Biology
    Journal of embryology and experimental morphology
  • 1981
Although cells of the mouse morula lack the blatant asymmetric distribution of organelles observed in rat cells, a long pulse of HRP to compact 8-cell mouse embryos revealed a distinct restricted localization of the enzyme not evident at earlier pre-compaction stages.