The fluorescence emission of the apo-glucose oxidase from Aspergillus niger as probe to estimate glucose concentrations.

@article{dAuria1999TheFE,
  title={The fluorescence emission of the apo-glucose oxidase from Aspergillus niger as probe to estimate glucose concentrations.},
  author={Sabato d'Auria and P. T. D. Herman and Mose' Rossi and Joseph R. Lakowicz},
  journal={Biochemical and biophysical research communications},
  year={1999},
  volume={263 2},
  pages={550-3}
}
We developed a new method of glucose sensing using an inactive form of glucose oxidase from Aspergillus niger. Glucose oxidase was rendered inactive by removal of the FAD cofactor. The resulting apo-glucose oxidase still binds glucose as observed from a decrease in its intrinsic tryptophan fluorescence. 8-Anilino-1-naphthalenesulfonic acid (ANS) was found to bind spontaneously to apo-glucose oxidase as seen from an enhancement of the ANS fluorescence. The steady state intensity of the bound ANS… CONTINUE READING

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