The flexibility of actin filaments as revealed by fluorescence resonance energy transfer. The influence of divalent cations.

@article{Nyitrai1999TheFO,
  title={The flexibility of actin filaments as revealed by fluorescence resonance energy transfer. The influence of divalent cations.},
  author={Mikl{\'o}s Nyitrai and G{\'a}bor Hild and J{\'o}zsef Bel{\'a}gyi and B{\'e}la Somogyi},
  journal={The Journal of biological chemistry},
  year={1999},
  volume={274 19},
  pages={12996-3001}
}
The temperature profile of the fluorescence resonance energy transfer efficiency normalized by the fluorescence quantum yield of the donor in the presence of acceptor, f', was measured in a way allowing the independent investigation of (i) the strength of interaction between the adjacent protomers (intermonomer flexibility) and (ii) the flexibility of the protein matrix within actin protomers (intramonomer flexibility). In both cases the relative increase as a function of temperature in f' is… CONTINUE READING