The fission yeast cdc19+ gene encodes a member of the MCM family of replication proteins.

Abstract

We have cloned and characterized the fission yeast cdc19+ gene. We demonstrate that it encodes a structural homologue of the budding yeast MCM2 protein. In fission yeast, the cdc19+ gene is constitutively expressed, and essential for viability. Deletion delays progression through S phase, and cells arrest in the first cycle with an apparent 2C DNA content, with their checkpoint control intact. The temperature-sensitive cdc19-P1 mutation is synthetically lethal with cdc21-M68. In addition, we show by classical and molecular genetics that cdc19+ is allelic to the nda1+ locus. We conclude that cdc19p plays a potentially conserved role in S phase.

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@article{Forsburg1994TheFY, title={The fission yeast cdc19+ gene encodes a member of the MCM family of replication proteins.}, author={Susan L. Forsburg and Paul Nurse}, journal={Journal of cell science}, year={1994}, volume={107 ( Pt 10)}, pages={2779-88} }