The first homolog of pacifastin-related precursor in the swimming crab (Portunus trituberculatus): characterization and potential role in immune response to bacteria and fungi.

@article{Wang2012TheFH,
  title={The first homolog of pacifastin-related precursor in the swimming crab (Portunus trituberculatus): characterization and potential role in immune response to bacteria and fungi.},
  author={Shuangyan Wang and Zhaoxia Cui and Yuan Hua Liu and QianQian Li and Chengwen Song},
  journal={Fish & shellfish immunology},
  year={2012},
  volume={32 2},
  pages={331-8}
}
Among all the serine proteinase inhibitor families (SPIs), the pacifastin-related inhibitor is seldom isolated. A pacifastin-related SPI named as PtPLC was identified from the swimming crab Portunus trituberculatus in this study. The full-length of PtPLC was cloned from haemocytes cDNA library by the combination of homology cloning and rapid amplification of cDNA ends (RACE). The PtPLC contained an open reading frame (ORF) of 1098 bp encoding a putative pacifastin-related precursor of 365 amino… CONTINUE READING