The first complete chloroplast genome sequence of a lycophyte, Huperzia lucidula (Lycopodiaceae).

@article{Wolf2005TheFC,
  title={The first complete chloroplast genome sequence of a lycophyte, Huperzia lucidula (Lycopodiaceae).},
  author={Paul G Wolf and Kenneth G Karol and Dina F. Mandoli and Jennifer V. Kuehl and Kathiravetpillai Arumuganathan and Mark W. Ellis and Brent D. Mishler and Dean G. Kelch and Richard G. Olmstead and Jeffrey L. Boore},
  journal={Gene},
  year={2005},
  volume={350 2},
  pages={117-28}
}
We used a unique combination of techniques to sequence the first complete chloroplast genome of a lycophyte, Huperzia lucidula. This plant belongs to a significant clade hypothesized to represent the sister group to all other vascular plants. We used fluorescence-activated cell sorting (FACS) to isolate the organelles, rolling circle amplification (RCA) to amplify the genome, and shotgun sequencing to 8x depth coverage to obtain the complete chloroplast genome sequence. The genome is 154,373 bp… CONTINUE READING
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