The first and second backcross progeny of the intergeneric fusion hybrids of potato and tomato after crossing with potato

  title={The first and second backcross progeny of the intergeneric fusion hybrids of potato and tomato after crossing with potato},
  author={Evert Jacobsen and Mousain Daniel and J. E. M. Bergervoet-van Deelen and Dirk Jan Huigen and Munikote S. Ramanna},
  journal={Theoretical and Applied Genetics},
Somatic fusion hybrids between the diploid potato and tomato were backcrossed to several genotypes of potato. Two ploidy levels of fusion hybrids, 4x and 6x, were used as female parents in backcrosses with five clones of 4x-potato. An estimate of the berry set and “seed set” in immature berries harvested 14–21 days after pollination indicated that crosses between certain combinations of 6x-fusion hybrids and male parents were more successful than others. The culture of over 4000 young seeds… 

Development of intermonoploid somatic hybrids of potato and their molecular analysis based on polymorphism for retroelement Tst1

Sequence specific amplified polymorphism (S-SAP) represents a hybrid system incorporating amplified fragment length polymorphisms (AFLP) technology in conjunction with the use of a defined genomic sequence, e.g., retrotransposon display (RD) when the defined sequence is anchored into a consensus sequence of a retrotransposition such as the long terminal repeat (LTR) sequence of Tst1.

Establishment of a complete series of a monosomic tomato chromosome addition lines in the cultivated potato using RFLP and GISH analyses

The present study showed that molecular markers and molecular cytogenetics applied in this study were most efficient and rapid because a pre-selection for the desired genotypes was possible by screening a population with chromosome-specific markers for the presence of one tomato chromosome at a time.

Intergeneric transfer of a partial genome and direct production of monosomic addition plants by microprotoplast fusion

The results suggest that the transfer of single chromosomes is tolerated better than is theTransfer of the whole donor genome, and the direct production of monosomic addition lines for the transfer and introgression of economically important traits in sexually-incongruent species is discussed.

Somatic hybrids of Solanum tuberosum and species of the Solanum nigrum-complex and their backcross progeny

It was shown that the ploidy level as well as the genotype were factors that influenced the somatic combining abilities, and the application of genomic in situ hybridisation (GISH) made it possible to distinguish clearly between S. nigrum and potato chromosomes in mitotic and meiotic chromosome spreads.

Somatic hybridization between Solanum tuberosum and species of the S. nigrum complex: Selection of vigorously growing and flowering plants

The use of kanamycin or hygromycin resistance as cell-selectable markersystem had no influence on somatic combining ability, but such markers can be useful to improve efficient selection of somatic hybrids in sufficient numbers.

Production and characterization of “second generation” somatic hybrids derived from protoplast fusion between interspecific somatohaploid and dihaploidSolanum tuberosum L.

Most of the “second generation” somatic hybrids were vigorous, but variable in morphology and they were extremely resistant to PLRV and they had tolerance to PVY infection derived from the somatohaploid fusion partner.

The potential of somatic hybridization in crop breeding

The potential of somatic hybridization in the breeding of crops within the Brassicaceae, Fabaceae, Poaceae and Solanaceae is discussed and it is evident from these studies that many hybrids, either symmetric or asymmetric, which are fertile have the potential to be used as a bridge between the alien species and the crop.

Somatic hybridization between Solanum ochranthum and Lycopersicon esculentum

Somatic hybridization methods were developed to facilitate the use of S. ochranthum for tomato germplasm improvement and hybridity was established by morphological characters, peroxidase isozyme and RAPD markers.

The impact on biosafety of the phosphinothricin-tolerance transgene in inter-specific B. rapa×B. napus hybrids and their successive backcrosses

The transfer of a phosphinothricin-tolerance transgene by inter-specific hybridization between B. rapa and two transgenic B. napus lines was investigated and it was found that the line showing least transmission during backcrossing contains a transGene integrated into a C-genome chromosome.

The use of molecular cytogenetic methods in the investigation of distant potato hybrids

This paper reviews the results of studies of interspecies hybridization, polyploidization, as well as phylogenetic relationships of Solanum species and members of closely related taxa by such



Isolation and characterization of potato-tomato somatic hybrids using an amylose-free potato mutant as parental genotype

Although all fusion hybrids were totally sterile, the hexaploid produced stainable pollen and berries with badly developed seeds, and Embryo rescue has so far failed to produce backcross progeny.

Somatic hybrid plants of potato and tomato regenerated from fused protoplasts

In the absence of a selection system against the potato parent, the analysis of ribulose bisphosphate carboxylase provides a convenient marker to demonstrate the hybrid nature of the plants.

Isolation and characterisation of somatic hybrids of diploid Solanum tuberosum and Solanum brevidens and the use of amylose-free starch mutation for detection of introgression

In the backcross progeny of hexaploid F12-5 with a tetraploid amf mutant one amylose-free recombinant among 67 plants was found, indicating the occurrence of meiotic recombination in the megaspore mother cells.

Limited DNA elimination from the irradiated potato parent in fusion products of albino Lycopersicon esculentum and Solanum tuberosum

The analysis of the elimination of potato DNA from potato-tomato somatic cell hybrids and the absence of true cybrids is described.

RFLP maps of potato and their alignment with the homoeologous tomato genome

Comparisons between potato RFLP maps derived from different genetic backgrounds revealed conservation of marker order but differences in chromosome and total map length, and significant reduction of map length was observed in interspecific compared to intraspecific crosses.

Phenotypic and genotypic characterization of an amylose-free starch mutant of the potato

Analysis of the F1 plants and of F1 × F1 progeny plants showed that the amf-character is monogenic and recessive, and the locus involved was designated amf+and the mutated allele amf.

Chilling resistance of somatic hybrids of tomato and potato

The chilling resistance of all four tomato-potato hybrids was intermediate between the chilling resistances of tomato and potato, and may be useful for transferring genes for chilling resistance into the domestic tomato.

Transformation of homozygous diploid potato with an Agrobacterium tumefaciens binary vector system by adventitious shoot regeneration on leaf and stem segments

Analysis of a number of randomly selected regenerated shoots for their ability to root and form shoots on kanamycin-containing medium shows that over 90% of the regenerated shoot obtained are transformed.

Successful hybridization of non-tuberous Solanum etuberosumLind. and tuberbearing S. pinnatisectumDun.

It is suggested that this hybrid together with those between the tomato, Lycopersicon esculentum, and S, pennellii and S. lycopersicoides constitute piers of a bridge between tomato and potato species which in the future might enable gene transfer between these two crops via their wild relatives.

Solanum lycopersicoides gene introgression to tomato, Lycopersicon esculentum, through the systematic avoidance and suppression of breeding barriers

SummaryWhile Lycopersicon esculentum and Solanum lycopersicoides have been successfully hybridized, attempts at further direct gene introgression have been unsuccessful due to the presence of