The fifth electron in the fully reduced caa(3) from Thermus thermophilus is competent in proton pumping.

@article{Siletsky2013TheFE,
  title={The fifth electron in the fully reduced caa(3) from Thermus thermophilus is competent in proton pumping.},
  author={Sergey A. Siletsky and Ilya Belevich and Tewfik Soulimane and Michael I. Verkhovsky and M{\aa}rten Wikstr{\"o}m},
  journal={Biochimica et biophysica acta},
  year={2013},
  volume={1827 1},
  pages={1-9}
}
The time-resolved kinetics of membrane potential generation coupled to oxidation of the fully reduced (five-electron) caa(3) cytochrome oxidase from Thermus thermophilus by oxygen was studied in a single-turnover regime. In order to calibrate the number of charges that move across the vesicle membrane in the different reaction steps, the reverse electron transfer from heme a(3) to heme a and further to the cytochrome c/Cu(A) has been resolved upon photodissociation of CO from the mixed valence… CONTINUE READING