The fertilization layer mediated block to polyspermy in Xenopus laevis: isolation of the cortical granule lectin ligand.

@article{Quill1996TheFL,
  title={The fertilization layer mediated block to polyspermy in Xenopus laevis: isolation of the cortical granule lectin ligand.},
  author={Timothy A. Quill and Jerry L. Hedrick},
  journal={Archives of biochemistry and biophysics},
  year={1996},
  volume={333 2},
  pages={326-32}
}
The fertilization layer of Xenopus laevis eggs is formed by the cortical granule lectin binding to its ligand. The binding requires Ca2+, is specific for galactose, and functionally establishes a block to polyspermy at fertilization. We have designed a new enzyme-linked lectin assay for the cortical granule lectin (CGL) ligand which can detect the presence of the CGL ligand at a sensitivity of 1-2 ng/ml. This assay is specifically inhibited with galactose, 50% inhibition at 9.9 mM, and produces… CONTINUE READING

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