The ferric reducing ability of plasma (FRAP) as a measure of "antioxidant power": the FRAP assay.

  title={The ferric reducing ability of plasma (FRAP) as a measure of "antioxidant power": the FRAP assay.},
  author={Iris Frances Forster Benzie and J. J. Strain},
  journal={Analytical biochemistry},
  volume={239 1},
A simple, automated test measuring the ferric reducing ability of plasma, the FRAP assay, is presented as a novel method for assessing "antioxidant power." Ferric to ferrous ion reduction at low pH causes a colored ferrous-tripyridyltriazine complex to form. FRAP values are obtained by comparing the absorbance change at 593 nm in test reaction mixtures with those containing ferrous ions in known concentration. Absorbance changes are linear over a wide concentration range with antioxidant… 

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