The fast and the slow: folding and trapping of λ6-85.

  title={The fast and the slow: folding and trapping of $\lambda$6-85.},
  author={Maxim B. Prigozhin and Martin Gruebele},
  journal={Journal of the American Chemical Society},
  volume={133 48},
Molecular dynamics simulations combining many microsecond trajectories have recently predicted that a very fast folding protein like lambda repressor fragment λ(6-85) D14A could have a slow millisecond kinetic phase. We investigated this possibility by detecting temperature-jump relaxation to 5 ms. While λ(6-85) D14A has no significant slow phase, two even more stable mutants do. A slow phase of λ(6-85) D14A does appear in mild denaturant. The experimental data and computational modeling… 

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