The extragenic spacer length between the 5' and 3' ends of the transgene expression cassette affects transgene silencing from plasmid-based vectors.

@article{Lu2012TheES,
  title={The extragenic spacer length between the 5' and 3' ends of the transgene expression cassette affects transgene silencing from plasmid-based vectors.},
  author={Jiamiao Lu and Feijie Zhang and Siqun Xu and Andrew Z Fire and Mark A. Kay},
  journal={Molecular therapy : the journal of the American Society of Gene Therapy},
  year={2012},
  volume={20 11},
  pages={2111-9}
}
In quiescent tissues, minicircle DNA vectors provide at least 10 times higher sustained levels of transgene expression compared to that achieved with a canonical plasmid containing the same expression cassette. It is not known if there is a specific DNA sequence or structure that is needed for DNA silencing. To directly address this question, we substituted… CONTINUE READING