The extraction and purification of microbial DNA from sediments

  title={The extraction and purification of microbial DNA from sediments},
  author={Andrew Ogram and Gary S. Sayler and Tamar Barkay},
A new method for the isolation of intracellular and extracellular DNA from a range of sediment types has been developed. This method is based upon the direct lysis of cells in the sediment, extraction of released DNA from the sediments and its subsequent purification by CsC1-EtBr gradient centrifugation and/or hydroxyapatite chromatography. Yields of 26 ~g intracellular DNA and 1 #g extracellular DNA have been obtained per gram of sediment. 
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