The externally derived portion of the hyperosmotic shock-activated cytosolic calcium pulse mediates adaptation to ionic stress in suspension-cultured tobacco cells.

  title={The externally derived portion of the hyperosmotic shock-activated cytosolic calcium pulse mediates adaptation to ionic stress in suspension-cultured tobacco cells.},
  author={Stephen G. Cessna and Tracie K. Matsumoto and Gregory N Lamb and Shawn J. Rice and Wendy Wenger Hochstedler},
  journal={Journal of plant physiology},
  volume={164 7},
The influx of Ca(2+) into the cytosol has long been suggested to serve as a signaling intermediate in the acquisition of tolerance to hyperosmotic and/or salinity stresses. Here we use aequorin-transformed suspension-cultured tobacco cells to directly assess the role of cytosolic calcium (Ca(2+)(cyt)) signaling in salinity tolerance acquisition. Aequorin luminescence recordings and (45)Ca influx measurements using inhibitors of Ca(2+) influx (Gd(3+) and the Ca(2+)-selective chelator EGTA), and… 
Abiotic stress responses promote Potato virus A infection in Nicotiana benthamiana.
It is suggested that the sensing of abiotic stress, which leads to cellular responses, probably via Ca(2+) signalling, associated with enhanced virus infection, may lead to higher field crop losses.
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Neomycin Inhibition of (+)-7-Iso-Jasmonoyl-L-Isoleucine Accumulation and Signaling
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Several PLC members have been implicated in various cellular processes and signaling networks, triggered in response to a number of environmental cues and developmental events in different plant species, which makes them potential candidates for genetically engineering the crop plants for stress tolerance and enhancing the crop productivity.
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Salt sensitivity of ranunculus, as determined by growth of the flowering stems and viability of the tuberous roots, was increased by irrigation with alkaline waters, which was associated with additional increases in Na + and Cl - tissue concentration and decreased iron accumulation.
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An Osmotically Induced Cytosolic Ca2+ Transient Activates Calcineurin Signaling to Mediate Ion Homeostasis and Salt Tolerance of Saccharomyces cerevisiae *
NaCl-induced ENA1expression was inhibited by EGTA, cch1Δ mutation, and FK506, indicating that the [Ca2+] cyt transient activates calcineurin signaling to mediate ion homeostasis and salt tolerance.
Hypo-osmotic Shock of Tobacco Cells Stimulates Ca2+Fluxes Deriving First from External and then Internal Ca2+Stores*
o-osmotic shock of aequorin-transformed tobacco cells induces a biphasic cytosolic Ca2+influx, and data suggest that osmotic shock leads to the sequential opening of extracellular followed by intracellular Ca2+.
Activation of the oxidative burst in aequorin-transformed Nicotiana tabacum cells is mediated by protein kinase- and anion channel-dependent release of Ca2+ from internal stores
It is hypothesized that the oxidative burst does not depend on the influx of external Ca2+, but instead may generally be mediated by the release of internal Ca2+ in a manner that depends on the proper function of kinases and anion channels.
Calcium signalling in Arabidopsis thaliana responding to drought and salinity.
Calcium transients were of a similar magnitude and duration in response to both mannitol and isoosmolar concentrations of salt, suggesting that a factor other than calcium is involved in the discrimination between drought and salinity signals in Arabidopsis.
Hyperosmotic stress induces a rapid and transient increase in inositol 1,4,5-trisphosphate independent of abscisic acid in Arabidopsis cell culture.
Analysis of changes in inositol 1,4,5-trisphosphate content in response to hyperosmotic shock or salinity in Arabidopsis thaliana T87 cultured cells suggests the involvement of PI-PLC and Ins(1, 4,5)P3 in an ABA-independent hyperOSmotic-stress signal transduction pathway in higher plants.
Inositol(1,4,5)trisphosphate production in plant cells: an early response to salinity and hyperosmotic stress
Data indicate that increased Ins(1,4,5)P3 production is a common response to salt and hyperosmotic stresses in plants and that it may play an important role in the processes leading to stress tolerance.
Regulation of ion homeostasis under salt stress.
  • J. Zhu
  • Biology, Medicine
    Current opinion in plant biology
  • 2003
Evidence suggests that aprotein kinase complex consisting of the myristoylated calcium-binding protein SOS3 and the serine/threonine protein kinase SOS2 is activated by a salt-stress-elicited calcium signal, which phosphorylates and activates various ion transporters, such as the plasma membrane Na(+)/H(+) antiporter SOS1.
Calcium in plants.
An overview of the nutritional requirements of different plants for Ca is provided, and how this impacts on natural flora and the Ca content of crops is reviewed.
Transgenic plant aequorin reports the effects of touch and cold-shock and elicitors on cytoplasmic calcium
It is shown here that aequorin can be reconstituted in transformed plants and that it reports calcium changes induced by touch, cold-shock and fungal elicitors, which could be valuable for determining the role of calcium in intracellular signalling processes in plants.
The Protein Kinase SOS2 Activates the Arabidopsis H+/Ca2+ Antiporter CAX1 to Integrate Calcium Transport and Salt Tolerance*
It is demonstrated that SOS2 regulates the vacuolar H+/Ca2+ antiporter CAX1, and this work suggests multiple functions for SOS2 and provides a mechanistic link between Ca2+ and Na+ homeostasis in plants.