The expression of tumor necrosis factor in human adipose tissue. Regulation by obesity, weight loss, and relationship to lipoprotein lipase.

@article{Kern1995TheEO,
  title={The expression of tumor necrosis factor in human adipose tissue. Regulation by obesity, weight loss, and relationship to lipoprotein lipase.},
  author={Philip A. Kern and Mehrnoosh Saghizadeh and John M. Ong and Ronald J. Bosch and Richard L. Deem and Rosa Simsolo},
  journal={The Journal of clinical investigation},
  year={1995},
  volume={95 5},
  pages={
          2111-9
        }
}
A previous study reported the increased expression of the cytokine TNF in the adipose tissue of genetically obese rodents. To examine this paradigm in humans, we studied TNF expression in lean, obese, and reduced-obese human subjects. TNF mRNA was demonstrated in human adipocytes and adipose tissue by Northern blotting and PCR. TNF protein was quantitated by Western blotting and ELISA in both adipose tissue and the medium surrounding adipose tissue. Using quantitative reverse transcriptase PCR… 

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