The expression of three desaturase genes of Spirulina platensis in Escherichia coli DH5α – Heterologous expression of Spirulina-desaturase genes

@article{Apiradee2004TheEO,
  title={The expression of three desaturase genes of Spirulina platensis in Escherichia coli DH5α – Heterologous expression of Spirulina-desaturase genes},
  author={Hongsthong Apiradee and Paithoonrangsarid Kalyanee and Prapugrangkul Pongsathon and Deshnium Patcharaporn and Sirijuntarut Matura and Subhudhi Sanjukta and Cheevadhanarak Supapon and Tanticharoen Morakot},
  journal={Molecular Biology Reports},
  year={2004},
  volume={31},
  pages={177-189}
}
The genes from a cyanobacterium - Spirulina platensis strain C1 - that encode the acyl-lipid desaturases (desC, desA and desD) involved in γ-linolenic (GLA) synthesis have been successfully expressed for the first time in Escherichia coli by employing a pTrcHisA expression system. In this report, the authors describe the expression of the three Spirulina N-terminal 6xHis-desaturases as well as the functional analysis of these recombinant proteins. The gene products of desC, desA and desD have… CONTINUE READING
3 Citations
12 References
Similar Papers

References

Publications referenced by this paper.
Showing 1-10 of 12 references

Comp . Biochem . Physiol . Biochem . Mol . Biol

  • N Murata, H Wada
  • 2001

Annu . Rev . Plant Physiol . Plant Mol . Biol

  • DR Tocher, MJ Leaver, PA Hodgson
  • 1998

Pro . Lipid . Res

  • H Wada, M Avelange-Macherel
  • 1998

Tibtech

  • P Gravel, O Golaz
  • 1997

γ -linolenic acid: metabolism and its roles in nutrition and medicine

  • NP Murata, P Deshnium, Y Tasaka
  • (Eds by Huang YS, & Mills DE) pp
  • 1996
2 Excerpts

Biochem . J

  • P Deshnium, L Mustady, DA Los, Z Gombos, N Murata
  • 1995

Principles of Biochemistry

  • AL Lehninger, DL Nelson, MM Cox
  • 2nd ed Worth Publisher,
  • 1993
1 Excerpt

Molecular cloning: A Laboratoty Manual

  • J Sambrook, EF Fritsch, T Maniatis
  • 1989
1 Excerpt

The Lipid Handbook

  • JL Harwood
  • (Eds by Gunstone FD, Harwood JL, & Padley FB) pp
  • 1988
2 Excerpts

Similar Papers

Loading similar papers…