• Biology
  • Published 2011

The expression and immunoprotection of the truncated dermonecrotic toxin of rabbit Bordetella bronchiseptica

@inproceedings{Bo2011TheEA,
  title={The expression and immunoprotection of the truncated dermonecrotic toxin of rabbit Bordetella bronchiseptica},
  author={Hu Bo},
  year={2011}
}
Dermonecrotic toxin(DNT) is identified as one of the main virulence factor of Bordetella bronchiseptica.To study the immunoprotection of the binding and catalytic regions of DNT,the N-terminal 1,612 bp(DNT1) and the C-terminal 973 bp(DNT3) fragments of the DNT were cloned into the pET-28a(+) and expressed in E.coli BL21(DE3),respectively.The expression of recombinant protein DNT1 and DNT3 were identified by SDS-PAGE and western blot analysis.High level antibody against DNT was induced in the… CONTINUE READING