The basic method for skin damage treatment, including chemical wounds, is a topical application of different agents. Their objective is to repair structure of the skin and its functions. All dressings for treating wounds are classified as biological, artificial and composites containing both synthetic and natural materials. There are many studies concerning application of chitosan, which is a derivate of natural polymer chitin, as a basis for topical materials to treat burns. However, data are rather limited about application of chitosan for treating acid burns. Thus, the aim of research is to study the morphological futures of skin regeneration after the chemical burn applying chitosan membranes. We performed the experiment on 60 young rats (3 months old) with the chemical burns of third-degree (IIIA degree) to study the morphofunctional features of skin regeneration. Later we applied the chitosan membranes on the burns. We carried out a histologic investigation on the biopsy specimens of wound to determine the morphological features of wound regeneration. The results confirmed that earlier granulation and epithelialization of the skin surface happened as the chitosan membrane was applied on the acid effected surface. The final result of the application of chitosan film is to achieve full epithelialization, preserve the structure of tissues beneath the burn and prevent getting scars.