The excited-state interaction of resazurin and resorufin with amines in aqueous solutions. Photophysics and photochemical reactions.

@article{Bueno2002TheEI,
  title={The excited-state interaction of resazurin and resorufin with amines in aqueous solutions. Photophysics and photochemical reactions.},
  author={Cl{\'a}udia Peixoto Bueno and Manuel Villegas and Sonia G Bertolotti and Carlos M Previtali and Miguel Guillermo Neumann and Maria Encinas},
  journal={Photochemistry and photobiology},
  year={2002},
  volume={76 4},
  pages={
          385-90
        }
}
The photophysics and photochemical behavior of the phenoxazin-3-one dyes, resazurin and resorufin, have been studied in aqueous solutions. The irradiation of resazurin in the presence of amines leads to deoxygenation of the N-oxide group, giving resorufin. This photoreaction is highly dependent on the amine structure and is efficient only in the presence of tertiary aliphatic amines. The absorption and fluorescence properties of these dyes are dependent on pH. At pH above 7.5 both dyes are in… CONTINUE READING
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