The evaluation of maté (Ilex paraguariensis) genetic toxicity in human lymphocytes by the cytokinesis-block in the micronucleus assay.

@article{Alves2008TheEO,
  title={The evaluation of mat{\'e} (Ilex paraguariensis) genetic toxicity in human lymphocytes by the cytokinesis-block in the micronucleus assay.},
  author={Rafael Jos{\'e} Vargas Alves and Geraldo Pereira Jotz and Viviane Souza do Amaral and Tiago Maeso Hermes Montes and H. S. Menezes and Helo{\'i}sa Helena Rodrigues de Andrade},
  journal={Toxicology in vitro : an international journal published in association with BIBRA},
  year={2008},
  volume={22 3},
  pages={
          695-8
        }
}
The present study evaluates the clastogenic and/or aneugenic potential of maté (Ilex paraguariensis) - previously tested for the presence of 48 organophosphorous pesticides - in the culture of human lymphocytes in the absence of exogenous metabolic activation. Peripheral blood was obtained once from three healthy female donors for lymphocyte cell cultures. The cultures were treated with maté infusion (filtered in sterilized sartorius filter with a 0.22 mm pore membrane), distilled water… Expand
Evaluation of the cyto- and genotoxic activity of yerba mate (Ilex paraguariensis) in human lymphocytes in vitro.
TLDR
It is found that yerba mate extract induced a concentration-dependent, statistically significant increase in the level of apoptotic and necrotic cells and a decrease in the nuclear division index (NDI) in human peripheral lymphocytes in vitro. Expand
Evaluation of mutagenicity and antimutagenicity of Ilex paraguariensis A. St.-Hil.: Aquifoliaceae infusion on Allium cepa assay.
TLDR
Results suggests that substances present in mate can act potentiating mutagenic and carcinogenic agents present in other compounds or perhaps damaging cells barriers from certain substances that harm the genetic material. Expand
Yerba mate (Ilex paraguariensis) inhibits lymphocyte activation in vitro.
TLDR
It was demonstrated that YM produces a reduction in the amount of activated cells under the stimulation of PHA, which means that it might be used in diseases with an inflammatory component. Expand
Acute toxicity and anti inflammatory effects of supercritical extracts of Ilex paraguariensis
TLDR
The results obtained may be very promising since they open new perspectives for the therapeutic use of supercritical extracts of I. paraguariensisor its preparations as anti-inflammatory agents. Expand
EFEITOS DA TEMPERATURA E DO MATE (Ilex paraguariensis) SOBRE O PROCESSO DE CARCINOGÊNESE DE ESÔFAGO EM RATOS WISTAR MACHOS.
TLDR
Data indicate that mate tea presented protective effects against DNA damage and esophageal and liver carcinogenesis induced by diethylnitrosamine plus thermal injury in male Wistar rats. Expand
Sodium tungstate (Na2WO4) exposure increases apoptosis in human peripheral blood lymphocytes
TLDR
Experiments indicate that tungstate increases apoptosis of PBL, alters cell cycle progression, reduces cytokine production, and therefore warrants further investigation. Expand
Evaluation of the protective effect of Ilex paraguariensis and Camellia sinensis extracts on the prevention of oxidative damage caused by ultraviolet radiation.
TLDR
Results show that the rats exposed to ultraviolet radiation presented DNA damage in blood and increased protein carbonylation and lipid peroxidation in skin, demonstrated by the antioxidant and antigenotoxic properties presented by these teas. Expand
Chemopreventive effects of mate against mouse mammary and colon carcinogenesis.
TLDR
It is suggested that post-initiation treatment with organic mate tea-like inhibited the development of colon and mammary carcinogenesis in a two-step medium-term mouse carcinogenesis model. Expand
Maté attenuates DNA damage and carcinogenesis induced by diethylnitrosamine and thermal injury in rat esophagus.
TLDR
A significant lower incidence of esophageal papillomas and liver adenomas and tumor multiplicity was observed in the animals previously treated with maté at week 20, suggesting protective effects against DNA damage and esophagal and liver carcinogenesis induced by DEN. Expand
Recent advances on Ilex paraguariensis research: minireview.
TLDR
Investigation studies in animals have provided strong evidence of anti-inflammatory effects of Ilex paraguariensis, notably protecting cigarette-induced lung inflammation acting on macrophage migration and inactivating matrix-metalloproteinase. Expand
...
1
2
...

References

SHOWING 1-10 OF 18 REFERENCES
Mutagenic and genotoxic effects of mate (Ilex paraguariensis) in prokaryotic organisms.
TLDR
Oxygen reactive species may be present in mate solutions playing an essential role in its genotoxicity, with a maximal phage induction at concentrations of 10 to 20 mg/plate. Expand
Nontoxic, mutagenic, and clastogenic activities of Mate-Chimarrão (Ilex paraguariensis).
TLDR
The results suggest that a high consumption of mate-chimarrão can potentiate carcinogenesis in the human oropharynx and esophagus. Expand
Micronuclei induced by aneugens and clastogens in mononucleate and binucleate cells using the cytokinesis block assay.
TLDR
Testing some typical aneugens and clastogens in whole blood lymphocyte cultures of four donors with the cytokinesis block micronucleus (CBMN) assay suggested that MN in mononucleates may be an interesting additional parameter in the CBMN assay. Expand
The in vitro micronucleus technique.
  • M. Fenech
  • Biology, Medicine
  • Mutation research
  • 2000
TLDR
In its current basic form the CBMN assay can provide the following measures of genotoxicity and cytotoxicity: chromosome breakage, chromosome loss, chromosome rearrangement, cell division inhibition, necrosis and apoptosis. Expand
The human lymphocyte micronucleus assay: a comparison between whole-blood and separated-lymphocyte cultures.
TLDR
The data suggest that whole-blood cultures may be considered a better experimental condition for the detection of MN induced by chemicals in vitro. Expand
Chromosomal changes: induction, detection methods and applicability in human biomonitoring.
TLDR
A number of findings support the hypothesis of a predictive association between the frequency of MN in cytokinesis-blocked lymphocytes and cancer development. Expand
Identification of aneuploidy‐inducing agents using cytokinesis‐blocked human lymphocytes and an antikinetochore antibody
TLDR
Results indicate that this relatively simple assay can discriminate between aneuploidogens and clastogens and may allow a more rapid identification of environmental and therapeutic agents with aneuPLoidy‐inducing potential. Expand
HUMN project: detailed description of the scoring criteria for the cytokinesis-block micronucleus assay using isolated human lymphocyte cultures.
TLDR
Criteria for scoring micron nuclei and nucleoplasmic bridges in binucleated cells in the cytokinesis-block micronucleus assay for isolated human lymphocyte cultures are described in detail and will assist in the development of a procedure for calibrating scorers and laboratories so that results from different laboratories may be more comparable in the future. Expand
Maté: a risk factor for oral and oropharyngeal cancer.
TLDR
There is evidence in the literature that Maté consumption is in itself carcinogenic and plays a role in the development of cancers of the oral cavity and oropharynx and available information suggests that Mate drinking should be considered one of the risk factors for oral and opharyngeal cancer. Expand
Importance of detecting numerical versus structural chromosome aberrations.
TLDR
The aim is to compare the advantages and disadvantages of the in vitro micronucleus test to assess aneuploidy/polyploidsy in vitro and to review briefly the key questions related to aneuPLoidy and polyploids. Expand
...
1
2
...