The evaluation of a PCR-based method for identification of Salmonella enterica serotypes from environmental samples and various food matrices.

@article{Beaubrun2012TheEO,
  title={The evaluation of a PCR-based method for identification of Salmonella enterica serotypes from environmental samples and various food matrices.},
  author={Junia Jean-Gilles Beaubrun and Chorng-Ming Cheng and Kai-Shun Chen and Laura Anne Ewing and Hua Wang and Maria C Agpaoa and Mei-Chiung Jo Huang and Erin E Dickey and Jamie M Du and Donna M. Williams-Hill and Brittany N. Hamilton and Shirley A Micallef and Rachel E. Rosenberg Goldstein and Ashish Bino George and Sam W. Joseph and Amy R Sapkota and Andrew Patrick Jacobson and Ben D Tall and Mahendra H. Kothary and K Strickland Dudley and Darcy E. Hanes},
  journal={Food microbiology},
  year={2012},
  volume={31 2},
  pages={
          199-209
        }
}
The most commonly used method for serotyping Salmonella spp. is based on the Kaufmann-White scheme, and is composed of serological reactions using antibodies to LPS agglutinins. The multiplex PCR used in this investigation was established by Kim et al. to serotype the 30 most common clinical Salmonella serotypes, as determined by CDC. The PCR assay consists of two five-plex reactions and a single two-plex PCR reaction, based on six genetic loci from Salmonella enterica serotype Typhimurium and… CONTINUE READING
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