The estimation of host antigen in experimental and commercial influenza subunit preparations by delayed-type hypersensitivity reaction.

@article{Brand1983TheEO,
  title={The estimation of host antigen in experimental and commercial influenza subunit preparations by delayed-type hypersensitivity reaction.},
  author={ColinM. Brand and Foo Yew Eddy Liew},
  journal={Journal of biological standardization},
  year={1983},
  volume={11 4},
  pages={
          313-21
        }
}
A delayed-type hypersensitivity (DTH) test has been used to detect the presence of host-specific antigen(s) in highly-purified egg-grown influenza virus and virus components. Cyclophosphamide-treated mice, primed with an extract from allantoic fluid, showed DTH reactions when challenged with whole virus, spikeless virus particles or polymer-type subunits. All commercial egg-grown influenza vaccines showed the presence of host antigen, whereas lipid-free derivatives such as internal virus… CONTINUE READING
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