The essential yeast RNA binding protein Np13p is methylated.

  title={The essential yeast RNA binding protein Np13p is methylated.},
  author={Christian W Siebel and C. Guthrie},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  volume={93 24},
Arginine methylation is a prevalent modification found in many RNA binding proteins, yet little is known about its functional consequences. Using a monoclonal antibody, 1E4, we have shown that the yeast NPL3 gene product Np13p, an essential RNA binding protein with repeated RGG motifs, is arginine-methylated in vivo. The 1E4 epitope can be generated by incubating recombinant Np13p with partially purified bovine arginine methyltransferase block this reaction. Np13p methylation requires S… CONTINUE READING

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