The essential tyrosine‐containing loop conformation and the role of the C‐terminal multi‐helix region in eukaryotic phenylalanine ammonia‐lyases
@article{Pilbk2006TheET, title={The essential tyrosine‐containing loop conformation and the role of the C‐terminal multi‐helix region in eukaryotic phenylalanine ammonia‐lyases}, author={Sarolta Pilb{\'a}k and A. Tomin and J. Rétey and L. Poppe}, journal={The FEBS Journal}, year={2006}, volume={273} }
Besides the post‐translationally cyclizing catalytic Ala‐Ser‐Gly triad, Tyr110 and its equivalents are of the most conserved residues in the active site of phenylalanine ammonia‐lyase (PAL, EC 4.3.1.5), histidine ammonia‐lyase (HAL, EC 4.3.1.3) and other related enzymes. The Tyr110Phe mutation results in the most pronounced inactivation of PAL indicating the importance of this residue. The recently published X‐ray structures of PAL revealed that the Tyr110‐loop was either missing (for… CONTINUE READING
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References
SHOWING 1-10 OF 93 REFERENCES
Crystal structure of phenylalanine ammonia lyase: multiple helix dipoles implicated in catalysis.
- Chemistry, Medicine
- Biochemistry
- 2004
- 166
An active site homology model of phenylalanine ammonia-lyase from Petroselinum crispum.
- Biology, Medicine
- European journal of biochemistry
- 2002
- 69
Crystal structure of histidine ammonia-lyase revealing a novel polypeptide modification as the catalytic electrophile.
- Chemistry, Medicine
- Biochemistry
- 1999
- 173
Structures of two histidine ammonia-lyase modifications and implications for the catalytic mechanism.
- Chemistry, Medicine
- European journal of biochemistry
- 2002
- 26
Characterization of the active site of histidine ammonia-lyase from Pseudomonas putida.
- Chemistry, Medicine
- European journal of biochemistry
- 2001
- 55
L-phenylalanine ammonia-lyase. IV. Evidence that the prosthetic group contains a dehydroalanyl residue and mechanism of action.
- Chemistry, Medicine
- Archives of biochemistry and biophysics
- 1970
- 118
Structural Basis for the Entrance into the Phenylpropanoid Metabolism Catalyzed by Phenylalanine Ammonia-Lyase
- Biology, Medicine
- The Plant Cell Online
- 2004
- 219
- PDF
A phenylalanine ammonia‐lyase gene from parsley: structure, regulation and identification of elicitor and light responsive cis‐acting elements.
- Biology, Medicine
- The EMBO journal
- 1989
- 454
Structural and catalytic properties of the four phenylalanine ammonia-lyase isoenzymes from parsley (Petroselinum crispum Nym.).
- Biology, Medicine
- European journal of biochemistry
- 1994
- 114
Autocatalytic peptide cyclization during chain folding of histidine ammonia-lyase.
- Chemistry, Medicine
- Structure
- 2002
- 53