The essential carboxyl group in subunit c of the F1F0 ATP synthase can be moved and H(+)-translocating function retained.

@article{Miller1990TheEC,
  title={The essential carboxyl group in subunit c of the F1F0 ATP synthase can be moved and H(+)-translocating function retained.},
  author={Marvin J Miller and Martin Oldenburg and Robert H. Fillingame},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1990},
  volume={87 13},
  pages={
          4900-4
        }
}
The proteolipid subunit c of F1F0-type H(+)-transporting ATP synthases [ATP phosphohydrolase (H(+)-transporting), EC 3.6.1.34] contains a conserved Asp/Glu residue that is thought to function in H+ translocation. To test the importance of the position of this residue in the Escherichia coli enzyme, we used oligonucleotide-directed mutagenesis to move the carboxyl side chain from position 61 to position 58, 60, or 62. Mutant cells with these changes were incapable of growth via oxidative… CONTINUE READING
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