The epithelial sodium channel (ENaC) traffics to apical membrane in lipid rafts in mouse cortical collecting duct cells.

@article{Hill2007TheES,
  title={The epithelial sodium channel (ENaC) traffics to apical membrane in lipid rafts in mouse cortical collecting duct cells.},
  author={Warren G Hill and Michael B. Butterworth and Huamin Wang and Robert S. Edinger and Jonathan Lebowitz and Kathryn W. Peters and Raymond A Frizzell and J Patrick Johnson},
  journal={The Journal of biological chemistry},
  year={2007},
  volume={282 52},
  pages={37402-11}
}
We previously showed that ENaC is present in lipid rafts in A6 cells, a Xenopus kidney cell line. We now demonstrate that ENaC can be detected in lipid rafts in mouse cortical collecting duct ((MPK)CCD(14)) cells by detergent insolubility, buoyancy on density gradients using two distinct approaches, and colocalization with caveolin 1. Less than 30% of ENaC subunits were found in raft fractions. The channel subunits also colocalized on sucrose gradients with known vesicle targeting and fusion… CONTINUE READING

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