The energetic cost of protein synthesis in isolated hepatocytes of rainbow trout (Oncorhynchus mykiss)

@article{Pannevis1992TheEC,
  title={The energetic cost of protein synthesis in isolated hepatocytes of rainbow trout (Oncorhynchus mykiss)},
  author={Marinus C. Pannevis and Dominic F. Houlihan},
  journal={Journal of Comparative Physiology B},
  year={1992},
  volume={162},
  pages={393-400}
}
To establish the energetic cost of protein synthesis, isolated trout hepatocytes were used to measure protein synthesis and respiration simultaneously at a variety of temperatures. The presence of bovine serum albumin was essential for the viability of isolated hepatocytes during isolation, but, in order to measure protein synthesis rates, oxygen consumption rates and RNA-to-protein ratios, BSA had to be washed from the cells. Isolated hepatocytes were found to be capable of protein synthesis… CONTINUE READING