The endogenous cannabinoid, anandamide, inhibits dopamine transporter function by a receptor-independent mechanism.

@article{Oz2010TheEC,
  title={The endogenous cannabinoid, anandamide, inhibits dopamine transporter function by a receptor-independent mechanism.},
  author={Murat Oz and Vanaja Jaligam and Sehamuddin Galadari and George A Petroianu and Yaroslav M. Shuba and Toni Shaun Shippenberg},
  journal={Journal of neurochemistry},
  year={2010},
  volume={112 6},
  pages={1454-64}
}
The endocannabinoid, anandamide (AEA), modulates the activity of the dopamine transporter (DAT) in heterologous cells and synaptosomal preparations. The cellular mechanisms mediating this effect are unknown. The present studies employed live cell imaging techniques and the fluorescent, high affinity DAT substrate, 4-(4-(dimethylamino)-styryl)-N-methylpyridinium (ASP(+)), to address this issue. AEA addition to EM4 cells expressing yellow fluorescent protein-tagged human DAT (hDAT) produced a… CONTINUE READING