The efficient elimination of solid tumor cells by EGFR-specific and HER2-specific scFv-SNAP fusion proteins conjugated to benzylguanine-modified auristatin F.

@article{Woitok2016TheEE,
  title={The efficient elimination of solid tumor cells by EGFR-specific and HER2-specific scFv-SNAP fusion proteins conjugated to benzylguanine-modified auristatin F.},
  author={Mira Woitok and Diana Klose and J. Niesen and W. Richter and M. Abbas and C. Stein and Rolf Fendel and Magdalena Bialon and C. Puettmann and R. Fischer and S. Barth and K. Kolberg},
  journal={Cancer letters},
  year={2016},
  volume={381 2},
  pages={
          323-30
        }
}
  • Mira Woitok, Diana Klose, +9 authors K. Kolberg
  • Published 2016
  • Medicine, Biology
  • Cancer letters
  • Antibody-drug conjugates (ADCs) combine the potency of cytotoxic drugs with the specificity of monoclonal antibodies (mAbs). Most ADCs are currently generated by the nonspecific conjugation of drug-linker reagents to certain amino acid residues in mAbs, resulting in a heterogeneous product. To overcome this limitation and prepare ADCs with a defined stoichiometry, we use SNAP-tag technology as an alternative conjugation strategy. This allows the site-specific conjugation of O(6)-benzylguanine… CONTINUE READING

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