The efficient and rapid import of a peptide into primary B and T lymphocytes and a lymphoblastoid cell line.

@article{Fenton1998TheEA,
  title={The efficient and rapid import of a peptide into primary B and T lymphocytes and a lymphoblastoid cell line.},
  author={Matthew J. Fenton and N. Bone and Alison J. Sinclair},
  journal={Journal of immunological methods},
  year={1998},
  volume={212 1},
  pages={41-8}
}
Lymphocytes are notoriously difficult to transfect. The favoured technique, electroporation, has three major disadvantages: it is highly disruptive, causing large scale cell death; it is inefficient; quiescent primary lymphocytes are refractory to electroporation unless they have been partially activated. We have investigated the cellular import of the third helix of the Antennapedia homeodomain protein (pAntp) as an alternative method for introducing peptides into primary lymphocytes and… CONTINUE READING

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