The effects of some antibiotics on sheep lens glucose 6-phosphate dehydrogenase in vitro.

@article{Beydemir2003TheEO,
  title={The effects of some antibiotics on sheep lens glucose 6-phosphate dehydrogenase in vitro.},
  author={Ş{\"u}kr{\"u} Beydemir and Destan Nil Kulaçoğlu and F Cifti and I Kfrevioglu},
  journal={European journal of ophthalmology},
  year={2003},
  volume={13 2},
  pages={155-161}
}
PURPOSE To investigate the in vitro effects of gentamicin sulfate, vancomycin hydrochloride, sodium cefazolin and ceftriaxone on glucose 6-phosphate dehydrogenase enzyme (G6PD) purified from sheep lenses. METHODS G6PD was purified from sheep lenses with a yield of 66.8% and a specific activity of 7.8 U/mg proteins, and 10,400-fold using ammonium sulfate fractionation and 2,5-ADP Sepharose 4B affinity gel. The enzyme activity was determined by Beutlers method. RESULTS Gentamicin sulfate and… CONTINUE READING