The effects of plasmid copy number and sequence context upon transfection efficiency.

@article{Walker2004TheEO,
  title={The effects of plasmid copy number and sequence context upon transfection efficiency.},
  author={Wendy E Walker and David J. Porteous and A. C. Boyd},
  journal={Journal of controlled release : official journal of the Controlled Release Society},
  year={2004},
  volume={94 1},
  pages={245-52}
}
It is known that large P1 artificial chromosome (PAC) vectors exhibit reduced transfection efficiency in comparison to small plasmid vectors. We investigated the dynamics of this effect, by comparing expression from a small plasmid (4.7 kb) and a PAC vector (111 kb) containing the Enhanced Green Fluorescent Protein (EGFP) reporter gene under the control of a P(CMV) promoter. EGFP expression was detected by fluorescence activated cell sorting (FACS). We found that the lower transfection… CONTINUE READING

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