The effects of phorbol ester and Ca ionophore on c-fos and c-myc expression and on DNA synthesis in human lymphocytes are not directly related.

Abstract

Phorbol ester activates human lymphocyte proliferation as measured by [3H]thymidine incorporation and blast transformation. Phorbol ester and calcium ionophore A 23187 induce c-fos and c-myc expression in these cells as shown by the measure of the specific mRNA levels and of the c-fos nuclear protein amount. The combination of these two agents has a synergistic effect on c-fos but not on c-myc expression. Lymphocyte stimulation by phorbol ester for a short time induces c-fos and c-myc expression, but has no effect on [3H]thymidine incorporation. This result indicates that the induction of c-fos and c-myc expression is not sufficient to commit these cells to DNA synthesis.

Cite this paper

@article{Pompidou1987TheEO, title={The effects of phorbol ester and Ca ionophore on c-fos and c-myc expression and on DNA synthesis in human lymphocytes are not directly related.}, author={A. Pompidou and Marisol Corral and Paul Michel and Nicole Defer and Jacques Kruh and Tom Curran}, journal={Biochemical and biophysical research communications}, year={1987}, volume={148 1}, pages={435-42} }